| Rank | City | 1990 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Columbus | 636,323 | 1.2% |
| 2 | Cleveland | 505,450 | -1.3% |
| 3 | Cincinnati | 364,553 | -0.6% |
| 4 | Toledo | 332,832 | -0.6% |
| 5 | Akron | 223,182 | -0.6% |
| 6 | Dayton | 182,393 | -1.1% |
| 7 | Youngstown | 95,695 | – |
| 8 | Parma | 87,650 | – |
| 9 | Canton | 84,047 | – |
| 10 | Lorain | 71,326 | – |
| 11 | Springfield | 70,718 | – |
| 12 | Hamilton | 61,345 | – |
| 13 | Kettering | 60,410 | – |
| 14 | Lakewood | 59,649 | – |
| 15 | Elyria | 56,628 | – |
| 16 | Euclid | 54,864 | – |
| 17 | Cleveland Heights | 54,087 | – |
| 18 | Middletown | 51,863 | – |
| 19 | Mansfield | 50,925 | – |
| 20 | Warren | 50,890 | – |
| 21 | Cuyahoga Falls | 48,867 | – |
| 22 | Mentor | 47,539 | – |
| 23 | Lima | 45,140 | – |
| 24 | Newark | 44,902 | – |
| 25 | Fairfield | 39,704 | – |
| 26 | Huber Heights | 38,675 | – |
| 27 | Marion | 36,227 | – |
| 28 | Findlay | 36,037 | – |
| 29 | Strongsville | 35,305 | – |
| 30 | Lancaster | 34,570 | – |
| 31 | Upper Arlington | 34,292 | – |
| 32 | North Olmsted | 34,206 | – |
| 33 | Beavercreek | 33,579 | – |
| 34 | East Cleveland | 33,092 | – |
| 35 | Garfield Heights | 31,736 | – |
| 36 | Massillon | 31,136 | – |
| 37 | Fairborn | 30,953 | – |
| 38 | Shaker Heights | 30,928 | – |
| 39 | Westerville | 30,901 | – |
| 40 | Sandusky | 29,571 | – |
| 41 | Trotwood | 29,432 | – |
| 42 | Bowling Green | 28,867 | – |
| 43 | Kent | 28,852 | – |
| 44 | Brunswick | 28,440 | – |
| 45 | Barberton | 27,821 | – |
| 46 | Stow | 27,652 | – |
| 47 | Zanesville | 27,226 | – |
| 48 | Maple Heights | 27,089 | – |
| 49 | Westlake | 27,014 | – |
| 50 | Reynoldsburg | 26,162 | – |
| 51 | Riverside | 25,362 | – |
| 52 | Xenia | 24,902 | – |
| 53 | South Euclid | 24,188 | – |
| 54 | Gahanna | 24,009 | – |
| 55 | Norwood | 23,606 | – |
| 56 | Portsmouth | 23,514 | – |
| 57 | Alliance | 23,414 | – |
| 58 | North Royalton | 23,184 | – |
| 59 | Wooster | 23,075 | – |
| 60 | Brook Park | 22,866 | – |
| 61 | Steubenville | 22,235 | – |
| 62 | Chillicothe | 22,116 | – |
| 63 | Ashtabula | 21,688 | – |
| 64 | Parma Heights | 21,627 | – |
| 65 | North Ridgeville | 21,546 | – |
| 66 | Athens | 21,310 | – |
| 67 | Centerville | 21,276 | – |
| 68 | Eastlake | 21,093 | – |
| 69 | Niles | 20,985 | – |
| 70 | Oxford | 20,864 | – |
| 71 | Piqua | 20,759 | – |
| 72 | Ashland | 20,628 | – |
| 73 | Whitehall | 20,557 | – |
| 74 | Willoughby | 20,466 | – |
| 75 | Rocky River | 20,416 | – |
| 76 | Delaware | 20,245 | – |
| 77 | Mayfield Heights | 19,844 | – |
| 78 | Grove City | 19,586 | – |
| 79 | Troy | 19,540 | – |
| 80 | Medina | 19,269 | – |
| 81 | Green | 19,134 | – |
| 82 | Berea | 19,047 | – |
| 83 | Sidney | 18,957 | – |
| 84 | Tiffin | 18,724 | – |
| 85 | Solon | 18,636 | – |
| 86 | Forest Park | 18,552 | – |
| 87 | Oregon | 18,362 | – |
| 88 | Miamisburg | 18,146 | – |
| 89 | Fairview Park | 17,981 | – |
| 90 | Fremont | 17,874 | – |
| 91 | Sylvania | 17,455 | – |
| 92 | Hudson Village | 17,108 | – |
| 93 | Dublin | 17,021 | – |
| 94 | Bay Village | 17,000 | – |
| 95 | Defiance | 16,801 | – |
| 96 | New Philadelphia | 16,576 | – |
| 97 | Lyndhurst | 15,971 | – |
| 98 | Warrensville Heights | 15,869 | – |
| 99 | Maumee | 15,774 | – |
| 100 | Wadsworth | 15,719 | – |
In 1990, the largest city in Ohio was Columbus, with a population of 636,323. The second largest city is Cleveland, with a population of 505,450.
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