| Rank | City | 1840 Population |
|---|---|---|
| 1 | Cincinnati | 46,338 |
| 2 | Cleveland | 6,071 |
| 3 | Dayton | 6,067 |
| 4 | Columbus | 6,048 |
| 5 | Zanesville | 4,766 |
| 6 | Steubenville | 4,247 |
In 1840, the largest city in Ohio was Cincinnati, with a population of 46,338. The second largest city is Cleveland, with a population of 6,071.
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