| Rank | City | 2000 Population |
|---|---|---|
| 1 | Sudan | 1,043 |
| 2 | Daisetta | 1,039 |
| 3 | Tiki Island | 1,025 |
| 4 | Southmayd | 1,025 |
| 5 | Maud | 1,025 |
| 6 | Bandera | 1,008 |
| 7 | Kempner | 1,008 |
| 8 | Lakeside City | 991 |
| 9 | St. Jo | 976 |
| 10 | Krugerville | 966 |
| 11 | Chico | 956 |
| 12 | Gunter | 952 |
| 13 | Tom Bean | 956 |
| 14 | Northlake | 932 |
| 15 | Plum Grove | 936 |
| 16 | McLendon-Chisholm | 917 |
| 17 | Brownsboro | 912 |
| 18 | Copperas Cove | 30,024 |
| 19 | Briarcliff | 897 |
| 20 | New Fairview | 891 |
| 21 | Aurora | 887 |
| 22 | Selma | 862 |
| 23 | Jewett | 867 |
| 24 | Friendswood | 29,214 |
| 25 | Iowa Colony | 836 |
| 26 | Deer Park | 28,565 |
| 27 | Point | 799 |
| 28 | Lometa | 788 |
| 29 | Talty | 781 |
| 30 | Surfside Beach | 766 |
| 31 | Socorro | 27,766 |
| 32 | Fulshear | 737 |
| 33 | Matador | 748 |
| 34 | Strawn | 741 |
| 35 | Normangee | 728 |
| 36 | Lott | 724 |
| 37 | Campbell | 718 |
| 38 | Deport | 718 |
| 39 | Oak Grove | 717 |
| 40 | The Colony | 27,076 |
| 41 | Rio Vista | 695 |
| 42 | Roxton | 695 |
| 43 | Rule | 692 |
| 44 | San Perlita | 679 |
| 45 | Mountain City | 674 |
| 46 | Roby | 672 |
| 47 | Ladonia | 669 |
| 48 | Zavalla | 648 |
| 49 | Loraine | 650 |
| 50 | St. Paul | 636 |
| 51 | Colmesneil | 638 |
| 52 | Cushing | 637 |
| 53 | Pine Forest | 633 |
| 54 | Skellytown | 622 |
| 55 | Lancaster | 26,002 |
| 56 | Fate | 602 |
| 57 | Cross Roads | 602 |
| 58 | East Mountain | 590 |
| 59 | Weir | 573 |
| 60 | Hawk Cove | 571 |
| 61 | Snook | 570 |
| 62 | Rhome | 569 |
| 63 | Scurry | 569 |
| 64 | Talco | 569 |
| 65 | Annetta South | 558 |
| 66 | Lefors | 556 |
| 67 | Lone Oak | 539 |
| 68 | Ponder | 528 |
| 69 | Mount Enterprise | 529 |
| 70 | Lincoln Park | 524 |
| 71 | Pflugerville | 24,055 |
| 72 | Nome | 516 |
| 73 | Leary | 521 |
| 74 | Ropesville | 516 |
| 75 | New Berlin | 512 |
| 76 | Briaroaks | 506 |
| 77 | Buffalo Springs | 495 |
| 78 | Turkey | 492 |
| 79 | Oak Ridge | 485 |
| 80 | University Park | 23,362 |
| 81 | Annetta North | 467 |
| 82 | Kendleton | 467 |
| 83 | Post Oak Bend City | 455 |
| 84 | Gustine | 456 |
| 85 | Highland Haven | 453 |
| 86 | Hackberry | 443 |
| 87 | Pattison | 447 |
| 88 | Windthorst | 443 |
| 89 | Denison | 22,721 |
| 90 | Christine | 439 |
| 91 | Howardwick | 439 |
| 92 | Webberville | 437 |
| 93 | Alba | 432 |
| 94 | Lipan | 432 |
| 95 | Dodd City | 423 |
| 96 | South Mountain | 410 |
| 97 | Uhland | 402 |
| 98 | Lavon | 395 |
| 99 | Barstow | 403 |
| 100 | Newark | 1,043 |
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