| Rank | City | 2020 Population | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Jackson | 152,846 | -1.3% | 
| 2 | Gulfport | 72,837 | 0.7% | 
| 3 | Southaven | 54,774 | 1.1% | 
| 4 | Biloxi | 49,456 | 1.2% | 
| 5 | Hattiesburg | 47,275 | 0.3% | 
| 6 | Olive Branch | 39,904 | 1.8% | 
| 7 | Tupelo | 37,800 | 0.9% | 
| 8 | Meridian | 34,832 | -1.7% | 
| 9 | Greenville | 29,441 | -1.5% | 
| 10 | Clinton | 27,909 | 1.0% | 
| 11 | Madison | 27,764 | 1.4% | 
| 12 | Pearl | 27,034 | 0.7% | 
| 13 | Horn Lake | 26,745 | 0.3% | 
| 14 | Oxford | 25,896 | 3.2% | 
| 15 | Brandon | 25,113 | 1.5% | 
| 16 | Starkville | 24,594 | 0.3% | 
| 17 | Ridgeland | 24,221 | 0.1% | 
| 18 | Columbus | 23,682 | 0.0% | 
| 19 | Pascagoula | 21,963 | -0.2% | 
| 20 | Vicksburg | 21,440 | -1.1% | 
| 21 | Gautier | 19,000 | 0.2% | 
| 22 | Ocean Springs | 18,413 | 0.5% | 
| 23 | Laurel | 17,146 | -0.8% | 
| 24 | Hernando | 17,072 | 1.9% | 
| 25 | Long Beach | 16,768 | 1.3% | 
| 26 | Clarksdale | 14,747 | -2.0% | 
| 27 | Corinth | 14,550 | -0.0% | 
| 28 | Greenwood | 14,391 | -0.5% | 
| 29 | Natchez | 14,202 | -1.1% | 
| 30 | D'Iberville | 12,686 | 2.9% | 
| 31 | Grenada | 12,625 | -0.4% | 
| 32 | Byram | 12,615 | 0.9% | 
| 33 | McComb | 12,353 | -0.3% | 
| 34 | Moss Point | 12,117 | -1.2% | 
| 35 | Picayune | 11,858 | 0.9% | 
| 36 | Brookhaven | 11,724 | -0.6% | 
| 37 | Petal | 11,430 | 0.9% | 
| 38 | Cleveland | 11,136 | -1.0% | 
| 39 | Canton | 10,790 | -2.0% | 
| 40 | Yazoo City | 10,309 | -1.0% | 
| 41 | Flowood | 10,230 | 2.7% | 
| 42 | West Point | 10,074 | -1.1% | 
| 43 | Diamondhead | 9,565 | – | 
| 44 | Indianola | 9,524 | -1.1% | 
| 45 | Bay St. Louis | 9,459 | 0.2% | 
| 46 | Booneville | 8,686 | -0.1% | 
| 47 | Senatobia | 8,094 | -0.1% | 
| 48 | New Albany | 7,591 | -0.6% | 
| 49 | Batesville | 7,415 | -0.1% | 
| 50 | Waveland | 7,158 | 1.1% | 
| 51 | Richland | 7,116 | 0.3% | 
| 52 | Kosciusko | 7,090 | -0.4% | 
| 53 | Philadelphia | 7,034 | -0.6% | 
| 54 | Holly Springs | 6,643 | -1.5% | 
| 55 | Amory | 6,588 | -1.0% | 
| 56 | Louisville | 6,053 | -0.9% | 
| 57 | Columbia | 5,949 | -1.0% | 
| 58 | Carthage | 5,738 | 1.2% | 
| 59 | Pass Christian | 5,736 | 2.2% | 
| 60 | Pontotoc | 5,598 | -0.0% | 
| 61 | Forest | 5,417 | -0.5% | 
| 62 | Ripley | 5,391 | -0.0% | 
| 63 | Saltillo | 4,920 | 0.3% | 
| 64 | Aberdeen | 4,900 | -1.3% | 
| 65 | Crystal Springs | 4,841 | -0.4% | 
| 66 | Florence | 4,567 | 1.0% | 
| 67 | Waynesboro | 4,552 | -1.0% | 
| 68 | Ellisville | 4,552 | 0.2% | 
| 69 | Winona | 4,457 | -1.2% | 
| 70 | Wiggins | 4,254 | -0.3% | 
| 71 | Fulton | 4,039 | 0.2% | 
| 72 | Leland | 3,980 | -1.2% | 
| 73 | Magee | 3,927 | -1.1% | 
| 74 | Houston | 3,793 | 0.5% | 
| 75 | Morton | 3,673 | 0.6% | 
| 76 | Hazlehurst | 3,605 | -1.1% | 
| 77 | Leakesville | 3,589 | 14.9% | 
| 78 | Water Valley | 3,306 | -0.3% | 
| 79 | Newton | 3,182 | -0.6% | 
| 80 | Baldwyn | 3,119 | -0.6% | 
| 81 | Iuka | 3,081 | 0.2% | 
| 82 | Tutwiler | 2,926 | -1.9% | 
| 83 | Lucedale | 2,888 | -0.1% | 
| 84 | Poplarville | 2,836 | -0.2% | 
| 85 | Verona | 2,799 | -0.7% | 
| 86 | Ruleville | 2,619 | -1.4% | 
| 87 | Macon | 2,558 | -0.8% | 
| 88 | Okolona | 2,500 | -0.7% | 
| 89 | Guntown | 2,414 | 1.5% | 
| 90 | Hollandale | 2,328 | -1.5% | 
| 91 | Collins | 2,252 | -1.4% | 
| 92 | Durant | 2,216 | -1.9% | 
| 93 | Mendenhall | 2,203 | -1.3% | 
| 94 | Farmington | 2,055 | -0.6% | 
| 95 | Shelby | 2,017 | -1.0% | 
| 96 | Union | 1,992 | 0.0% | 
| 97 | Quitman | 1,990 | -1.5% | 
| 98 | Eupora | 1,942 | -1.2% | 
| 99 | Moorhead | 1,941 | -2.1% | 
| 100 | Nettleton | 1,928 | -0.3% | 
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