| Rank | City | 2000 Population | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Jackson | 185,260 | -0.6% | 
| 2 | Gulfport | 71,457 | 1.0% | 
| 3 | Biloxi | 50,691 | 0.9% | 
| 4 | Hattiesburg | 46,104 | 0.2% | 
| 5 | Greenville | 41,404 | -1.0% | 
| 6 | Meridian | 41,307 | -0.2% | 
| 7 | Tupelo | 34,294 | 1.1% | 
| 8 | Southaven | 29,508 | 3.4% | 
| 9 | Vicksburg | 26,342 | -0.2% | 
| 10 | Pascagoula | 26,165 | 0.0% | 
| 11 | Columbus | 25,883 | -0.8% | 
| 12 | Clinton | 25,158 | 1.4% | 
| 13 | Pearl | 22,748 | 1.6% | 
| 14 | Starkville | 21,998 | 1.5% | 
| 15 | Olive Branch | 21,328 | 11.0% | 
| 16 | Clarksdale | 20,581 | -0.6% | 
| 17 | Ridgeland | 20,333 | 4.7% | 
| 18 | Horn Lake | 20,155 | 8.2% | 
| 19 | Madison | 18,978 | 9.1% | 
| 20 | Greenwood | 18,442 | -0.4% | 
| 21 | Natchez | 18,372 | -0.7% | 
| 22 | Laurel | 18,271 | -0.7% | 
| 23 | Brandon | 17,709 | 3.8% | 
| 24 | Long Beach | 17,263 | 0.9% | 
| 25 | Ocean Springs | 17,258 | 1.3% | 
| 26 | Gautier | 16,856 | 5.2% | 
| 27 | Moss Point | 15,833 | -1.1% | 
| 28 | Grenada | 14,823 | 0.1% | 
| 29 | Corinth | 14,062 | 0.2% | 
| 30 | Cleveland | 13,765 | -1.2% | 
| 31 | Oxford | 13,673 | 2.6% | 
| 32 | McComb | 13,325 | 0.0% | 
| 33 | Brookhaven | 12,962 | 2.2% | 
| 34 | Canton | 12,920 | 0.3% | 
| 35 | Yazoo City | 12,160 | -0.6% | 
| 36 | West Point | 12,128 | 3.3% | 
| 37 | Indianola | 12,010 | -0.0% | 
| 38 | Bay St. Louis | 11,423 | 3.4% | 
| 39 | Picayune | 10,544 | -0.1% | 
| 40 | Petal | 9,859 | 2.2% | 
| 41 | Booneville | 8,628 | 0.8% | 
| 42 | Hernando | 8,085 | 6.0% | 
| 43 | Waveland | 7,968 | 3.9% | 
| 44 | Holly Springs | 7,965 | 0.7% | 
| 45 | D'Iberville | 7,908 | 1.9% | 
| 46 | New Albany | 7,696 | 1.5% | 
| 47 | Amory | 7,592 | 0.6% | 
| 48 | Batesville | 7,465 | 1.1% | 
| 49 | Philadelphia | 7,379 | 0.5% | 
| 50 | Kosciusko | 7,367 | 0.3% | 
| 51 | Senatobia | 7,016 | 3.0% | 
| 52 | Louisville | 6,999 | -0.8% | 
| 53 | Columbia | 6,610 | -0.8% | 
| 54 | Pass Christian | 6,588 | 1.8% | 
| 55 | Aberdeen | 6,411 | -1.3% | 
| 56 | Richland | 6,247 | 4.2% | 
| 57 | Forest | 5,976 | 1.1% | 
| 58 | Crystal Springs | 5,865 | 0.1% | 
| 59 | Waynesboro | 5,760 | 1.1% | 
| 60 | Flowood | 5,568 | 7.2% | 
| 61 | Ripley | 5,486 | 0.8% | 
| 62 | Leland | 5,480 | -1.7% | 
| 63 | Winona | 5,425 | -1.1% | 
| 64 | Pontotoc | 5,390 | 0.7% | 
| 65 | Carthage | 4,628 | 1.6% | 
| 66 | Hazlehurst | 4,392 | 0.2% | 
| 67 | Magee | 4,197 | 1.4% | 
| 68 | Ellisville | 4,158 | 1.1% | 
| 69 | Houston | 4,061 | -0.1% | 
| 70 | Fulton | 3,899 | 1.5% | 
| 71 | Wiggins | 3,865 | 0.7% | 
| 72 | Water Valley | 3,703 | 0.2% | 
| 73 | Newton | 3,695 | -0.3% | 
| 74 | Morton | 3,484 | 1.2% | 
| 75 | Hollandale | 3,417 | -0.4% | 
| 76 | Saltillo | 3,395 | 5.6% | 
| 77 | Verona | 3,340 | 1.2% | 
| 78 | Baldwyn | 3,325 | 0.1% | 
| 79 | Ruleville | 3,221 | -0.1% | 
| 80 | Okolona | 3,058 | -0.7% | 
| 81 | Iuka | 3,053 | -0.9% | 
| 82 | Macon | 2,997 | 2.5% | 
| 83 | Durant | 2,931 | 0.2% | 
| 84 | Shelby | 2,911 | 0.4% | 
| 85 | Collins | 2,689 | 0.5% | 
| 86 | Belzoni | 2,659 | 0.4% | 
| 87 | Poplarville | 2,605 | -0.2% | 
| 88 | Moorhead | 2,561 | 0.7% | 
| 89 | Mendenhall | 2,557 | 0.3% | 
| 90 | Florence | 2,547 | 2.6% | 
| 91 | Lucedale | 2,486 | -0.9% | 
| 92 | Rolling Fork | 2,469 | 0.1% | 
| 93 | Quitman | 2,458 | -1.1% | 
| 94 | Rosedale | 2,420 | -0.6% | 
| 95 | Drew | 2,419 | 0.1% | 
| 96 | Tchula | 2,355 | 0.7% | 
| 97 | Eupora | 2,323 | -0.4% | 
| 98 | Shaw | 2,311 | -0.4% | 
| 99 | Lumberton | 2,248 | -0.9% | 
| 100 | Fayette | 2,223 | 1.8% | 
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