| Rank | City | 2020 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Jackson | 160,628 | -0.9% |
| 2 | Gulfport | 71,705 | 0.6% |
| 3 | Southaven | 55,780 | 1.5% |
| 4 | Biloxi | 46,212 | 0.5% |
| 5 | Hattiesburg | 45,863 | -0.0% |
| 6 | Olive Branch | 38,924 | 1.7% |
| 7 | Tupelo | 38,312 | 1.2% |
| 8 | Meridian | 36,347 | -1.4% |
| 9 | Greenville | 29,085 | -1.8% |
| 10 | Oxford | 28,122 | 4.5% |
| 11 | Horn Lake | 27,272 | 0.5% |
| 12 | Pearl | 26,510 | 0.6% |
| 13 | Madison | 25,661 | 0.7% |
| 14 | Starkville | 25,653 | 0.8% |
| 15 | Clinton | 24,440 | -0.3% |
| 16 | Brandon | 24,289 | 1.3% |
| 17 | Ridgeland | 24,104 | 0.0% |
| 18 | Columbus | 23,573 | -0.0% |
| 19 | Pascagoula | 21,699 | -0.3% |
| 20 | Vicksburg | 21,653 | -1.1% |
| 21 | Gautier | 18,490 | -0.0% |
| 22 | Laurel | 18,338 | -0.1% |
| 23 | Ocean Springs | 17,862 | 0.3% |
| 24 | Hernando | 16,399 | 1.7% |
| 25 | Long Beach | 16,023 | 0.9% |
| 26 | Clarksdale | 14,894 | -2.1% |
| 27 | Natchez | 14,615 | -0.9% |
| 28 | Corinth | 14,472 | -0.1% |
| 29 | Bay St. Louis | 14,034 | 4.7% |
| 30 | D'Iberville | 14,012 | 4.4% |
| 31 | Greenwood | 13,561 | -1.3% |
| 32 | Moss Point | 13,350 | -0.3% |
| 33 | McComb | 13,013 | 0.2% |
| 34 | Grenada | 12,219 | -0.8% |
| 35 | Canton | 12,094 | -1.0% |
| 36 | Brookhaven | 11,947 | -0.5% |
| 37 | Byram | 11,428 | -0.1% |
| 38 | Cleveland | 11,073 | -1.2% |
| 39 | Picayune | 10,904 | 0.0% |
| 40 | Yazoo City | 10,869 | -0.5% |
| 41 | Petal | 10,632 | 0.2% |
| 42 | West Point | 10,404 | -0.9% |
| 43 | Flowood | 9,380 | 2.0% |
| 44 | Indianola | 9,037 | -1.8% |
| 45 | New Albany | 8,753 | 1.0% |
| 46 | Booneville | 8,497 | -0.3% |
| 47 | Diamondhead | 8,048 | – |
| 48 | Holly Springs | 7,798 | 0.1% |
| 49 | Senatobia | 7,610 | -0.8% |
| 50 | Richland | 7,241 | 0.5% |
| 51 | Batesville | 7,218 | -0.4% |
| 52 | Philadelphia | 7,078 | -0.6% |
| 53 | Amory | 6,794 | -0.8% |
| 54 | Kosciusko | 6,720 | -1.1% |
| 55 | Pass Christian | 6,307 | 3.5% |
| 56 | Waveland | 6,302 | -0.2% |
| 57 | Pontotoc | 6,169 | 1.0% |
| 58 | Louisville | 5,983 | -1.1% |
| 59 | Columbia | 5,837 | -1.3% |
| 60 | Forest | 5,536 | -0.3% |
| 61 | Ripley | 5,273 | -0.3% |
| 62 | Aberdeen | 5,205 | -0.8% |
| 63 | Saltillo | 5,062 | 0.7% |
| 64 | Waynesboro | 4,805 | -0.5% |
| 65 | Carthage | 4,782 | -0.7% |
| 66 | Crystal Springs | 4,715 | -0.7% |
| 67 | Ellisville | 4,559 | 0.3% |
| 68 | Wiggins | 4,541 | 0.4% |
| 69 | Florence | 4,494 | 0.9% |
| 70 | Magee | 4,097 | -0.8% |
| 71 | Fulton | 3,980 | 0.1% |
| 72 | Winona | 3,964 | -2.6% |
| 73 | Leland | 3,766 | -1.9% |
| 74 | Hazlehurst | 3,730 | -0.8% |
| 75 | Morton | 3,527 | 0.2% |
| 76 | Houston | 3,427 | -0.6% |
| 77 | Tutwiler | 3,416 | -0.4% |
| 78 | Baldwyn | 3,268 | -0.1% |
| 79 | Water Valley | 3,255 | -0.5% |
| 80 | Verona | 3,232 | 0.8% |
| 81 | Newton | 3,155 | -0.7% |
| 82 | Lucedale | 3,153 | 0.8% |
| 83 | Iuka | 2,937 | -0.3% |
| 84 | Poplarville | 2,899 | 0.0% |
| 85 | Guntown | 2,792 | 3.3% |
| 86 | Okolona | 2,591 | -0.4% |
| 87 | Ruleville | 2,557 | -1.8% |
| 88 | Collins | 2,425 | -0.7% |
| 89 | Macon | 2,421 | -1.5% |
| 90 | Purvis | 2,392 | 1.1% |
| 91 | Mendenhall | 2,387 | -0.5% |
| 92 | Hollandale | 2,304 | -1.8% |
| 93 | Durant | 2,262 | -1.8% |
| 94 | Lumberton | 2,218 | 0.7% |
| 95 | Magnolia | 2,212 | -1.0% |
| 96 | Farmington | 2,155 | -0.2% |
| 97 | Raymond | 2,115 | 1.0% |
| 98 | Quitman | 2,111 | -1.1% |
| 99 | Moorhead | 2,063 | -1.7% |
| 100 | Eupora | 2,005 | -1.0% |
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