| Rank | City | 2016 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Jackson | 170,674 | -0.3% |
| 2 | Gulfport | 71,856 | 1.2% |
| 3 | Southaven | 52,589 | 1.4% |
| 4 | Hattiesburg | 46,805 | 0.4% |
| 5 | Biloxi | 45,637 | 0.7% |
| 6 | Meridian | 39,661 | -0.7% |
| 7 | Olive Branch | 36,010 | 1.5% |
| 8 | Tupelo | 35,680 | 0.6% |
| 9 | Greenville | 32,156 | -1.3% |
| 10 | Horn Lake | 26,915 | 0.6% |
| 11 | Pearl | 26,462 | 1.1% |
| 12 | Madison | 25,799 | 1.3% |
| 13 | Starkville | 25,366 | 1.2% |
| 14 | Clinton | 25,254 | 0.0% |
| 15 | Ridgeland | 24,351 | 0.3% |
| 16 | Brandon | 23,529 | 1.6% |
| 17 | Columbus | 23,168 | -0.4% |
| 18 | Vicksburg | 23,131 | -0.6% |
| 19 | Oxford | 22,314 | 3.4% |
| 20 | Pascagoula | 22,126 | -0.2% |
| 21 | Laurel | 18,837 | 0.3% |
| 22 | Gautier | 18,570 | -0.0% |
| 23 | Ocean Springs | 17,636 | 0.2% |
| 24 | Clarksdale | 16,847 | -1.3% |
| 25 | Long Beach | 15,555 | 1.0% |
| 26 | Hernando | 15,503 | 1.9% |
| 27 | Greenwood | 15,431 | 0.3% |
| 28 | Natchez | 15,128 | -0.9% |
| 29 | Corinth | 14,866 | 0.4% |
| 30 | Canton | 13,676 | 0.7% |
| 31 | Moss Point | 13,654 | -0.1% |
| 32 | Grenada | 12,900 | -0.3% |
| 33 | McComb | 12,661 | -0.2% |
| 34 | Brookhaven | 12,414 | -0.2% |
| 35 | Cleveland | 12,327 | -0.0% |
| 36 | Bay St. Louis | 12,030 | 5.4% |
| 37 | Byram | 11,509 | 0.0% |
| 38 | D'Iberville | 11,400 | 3.7% |
| 39 | Yazoo City | 11,245 | -0.3% |
| 40 | West Point | 10,990 | -0.6% |
| 41 | Petal | 10,701 | 0.5% |
| 42 | Picayune | 10,675 | -0.4% |
| 43 | Indianola | 9,943 | -1.4% |
| 44 | New Albany | 8,830 | 1.9% |
| 45 | Booneville | 8,816 | 0.2% |
| 46 | Flowood | 8,705 | 2.2% |
| 47 | Diamondhead | 8,132 | – |
| 48 | Senatobia | 7,963 | -0.5% |
| 49 | Holly Springs | 7,610 | -0.2% |
| 50 | Philadelphia | 7,391 | -0.2% |
| 51 | Batesville | 7,385 | -0.2% |
| 52 | Kosciusko | 7,187 | -0.6% |
| 53 | Richland | 7,087 | 0.5% |
| 54 | Amory | 7,067 | -0.7% |
| 55 | Waveland | 6,391 | -0.1% |
| 56 | Louisville | 6,314 | -1.0% |
| 57 | Columbia | 6,229 | -1.1% |
| 58 | Pontotoc | 5,944 | 1.1% |
| 59 | Forest | 5,695 | 0.0% |
| 60 | Pass Christian | 5,498 | 3.6% |
| 61 | Aberdeen | 5,397 | -0.8% |
| 62 | Ripley | 5,357 | -0.1% |
| 63 | Saltillo | 5,004 | 1.0% |
| 64 | Waynesboro | 4,975 | -0.3% |
| 65 | Crystal Springs | 4,939 | -0.4% |
| 66 | Carthage | 4,899 | -0.7% |
| 67 | Ellisville | 4,573 | 0.6% |
| 68 | Wiggins | 4,550 | 0.7% |
| 69 | Winona | 4,439 | -2.5% |
| 70 | Magee | 4,368 | -0.2% |
| 71 | Florence | 4,363 | 1.0% |
| 72 | Leland | 4,188 | -1.3% |
| 73 | Fulton | 4,077 | 0.6% |
| 74 | Hazlehurst | 3,924 | -0.4% |
| 75 | Houston | 3,544 | -0.4% |
| 76 | Tutwiler | 3,496 | -0.3% |
| 77 | Morton | 3,439 | -0.1% |
| 78 | Water Valley | 3,380 | -0.1% |
| 79 | Newton | 3,356 | -0.1% |
| 80 | Baldwyn | 3,339 | 0.3% |
| 81 | Verona | 3,054 | 0.3% |
| 82 | Lucedale | 3,011 | 0.6% |
| 83 | Iuka | 3,001 | -0.2% |
| 84 | Poplarville | 2,985 | 0.6% |
| 85 | Ruleville | 2,790 | -1.5% |
| 86 | Okolona | 2,638 | -0.4% |
| 87 | Macon | 2,616 | -1.1% |
| 88 | Guntown | 2,608 | 4.6% |
| 89 | Collins | 2,570 | -0.1% |
| 90 | Hollandale | 2,545 | -1.2% |
| 91 | Mendenhall | 2,488 | -0.1% |
| 92 | Durant | 2,486 | -1.4% |
| 93 | Magnolia | 2,371 | -0.4% |
| 94 | Purvis | 2,335 | 1.4% |
| 95 | Moorhead | 2,261 | -1.2% |
| 96 | Raymond | 2,225 | 2.9% |
| 97 | Lumberton | 2,223 | 1.3% |
| 98 | Quitman | 2,209 | -1.0% |
| 99 | Farmington | 2,194 | 0.1% |
| 100 | Shelby | 2,123 | -1.0% |
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