| Rank | City | 2009 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Jackson | 175,091 | -0.7% |
| 2 | Gulfport | 70,433 | -0.2% |
| 3 | Hattiesburg | 52,276 | 1.6% |
| 4 | Biloxi | 45,828 | -1.3% |
| 5 | Southaven | 44,135 | 5.2% |
| 6 | Meridian | 39,572 | -0.5% |
| 7 | Tupelo | 36,010 | 0.6% |
| 8 | Greenville | 35,614 | -1.9% |
| 9 | Olive Branch | 31,861 | 5.1% |
| 10 | Clinton | 26,244 | 0.5% |
| 11 | Vicksburg | 24,909 | -0.7% |
| 12 | Horn Lake | 24,749 | 2.6% |
| 13 | Pearl | 24,497 | 0.9% |
| 14 | Starkville | 23,992 | 1.1% |
| 15 | Pascagoula | 23,845 | -1.2% |
| 16 | Columbus | 23,755 | -1.1% |
| 17 | Brandon | 22,082 | 2.8% |
| 18 | Madison | 21,677 | 1.7% |
| 19 | Ridgeland | 21,588 | 0.8% |
| 20 | Laurel | 18,759 | 0.3% |
| 21 | Clarksdale | 17,916 | -1.7% |
| 22 | Ocean Springs | 17,318 | 0.0% |
| 23 | Oxford | 17,189 | 2.9% |
| 24 | Gautier | 16,525 | -0.2% |
| 25 | Natchez | 16,304 | -1.5% |
| 26 | Greenwood | 16,024 | -1.7% |
| 27 | Grenada | 14,690 | -0.1% |
| 28 | Corinth | 14,264 | 0.2% |
| 29 | Moss Point | 14,083 | -1.5% |
| 30 | McComb | 13,580 | 0.2% |
| 31 | Brookhaven | 13,130 | 0.2% |
| 32 | Canton | 12,542 | -0.4% |
| 33 | Hernando | 12,385 | 5.5% |
| 34 | Long Beach | 12,287 | -4.2% |
| 35 | Cleveland | 12,078 | -1.6% |
| 36 | Picayune | 11,875 | 1.5% |
| 37 | Yazoo City | 11,294 | -0.9% |
| 38 | West Point | 11,172 | -1.0% |
| 39 | Indianola | 10,709 | -1.4% |
| 40 | Petal | 10,697 | 1.0% |
| 41 | Booneville | 8,739 | 0.2% |
| 42 | Bay St. Louis | 8,148 | -4.1% |
| 43 | New Albany | 8,092 | 0.6% |
| 44 | D'Iberville | 7,969 | 0.1% |
| 45 | Holly Springs | 7,912 | -0.1% |
| 46 | Philadelphia | 7,823 | 0.7% |
| 47 | Batesville | 7,756 | 0.5% |
| 48 | Senatobia | 7,389 | 0.6% |
| 49 | Kosciusko | 7,330 | -0.1% |
| 50 | Richland | 7,328 | 2.0% |
| 51 | Amory | 7,204 | -0.7% |
| 52 | Flowood | 6,984 | 2.9% |
| 53 | Louisville | 6,536 | -0.9% |
| 54 | Columbia | 6,524 | -0.2% |
| 55 | Aberdeen | 6,050 | -0.7% |
| 56 | Forest | 6,027 | 0.1% |
| 57 | Crystal Springs | 5,916 | 0.1% |
| 58 | Pontotoc | 5,881 | 1.1% |
| 59 | Ripley | 5,708 | 0.5% |
| 60 | Waynesboro | 5,662 | -0.2% |
| 61 | Waveland | 5,291 | -5.0% |
| 62 | Wiggins | 4,924 | 3.1% |
| 63 | Leland | 4,772 | -1.7% |
| 64 | Carthage | 4,752 | 0.3% |
| 65 | Winona | 4,510 | -2.3% |
| 66 | Ellisville | 4,402 | 0.7% |
| 67 | Hazlehurst | 4,343 | -0.1% |
| 68 | Magee | 4,302 | 0.3% |
| 69 | Saltillo | 4,249 | 2.8% |
| 70 | Fulton | 4,043 | 0.5% |
| 71 | Pass Christian | 4,013 | -6.0% |
| 72 | Water Valley | 3,933 | 0.8% |
| 73 | Houston | 3,842 | -0.7% |
| 74 | Newton | 3,686 | -0.0% |
| 75 | Florence | 3,556 | 4.3% |
| 76 | Morton | 3,465 | -0.1% |
| 77 | Verona | 3,391 | 0.2% |
| 78 | Baldwyn | 3,313 | -0.0% |
| 79 | Lucedale | 3,064 | 2.6% |
| 80 | Poplarville | 3,022 | 1.9% |
| 81 | Hollandale | 2,954 | -1.8% |
| 82 | Iuka | 2,942 | -0.5% |
| 83 | Okolona | 2,847 | -0.9% |
| 84 | Ruleville | 2,826 | -1.6% |
| 85 | Collins | 2,776 | 0.4% |
| 86 | Macon | 2,719 | -1.2% |
| 87 | Lumberton | 2,703 | 2.3% |
| 88 | Durant | 2,694 | -1.0% |
| 89 | Purvis | 2,649 | 2.4% |
| 90 | Shelby | 2,594 | -1.4% |
| 91 | Mendenhall | 2,539 | -0.1% |
| 92 | Belzoni | 2,386 | -1.3% |
| 93 | Rosedale | 2,305 | -0.6% |
| 94 | Quitman | 2,302 | -0.8% |
| 95 | Moorhead | 2,300 | -1.3% |
| 96 | Farmington | 2,224 | 0.4% |
| 97 | Tchula | 2,211 | -0.8% |
| 98 | Eupora | 2,204 | -0.7% |
| 99 | Bay Springs | 2,180 | 0.4% |
| 100 | Drew | 2,156 | -1.4% |
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