| Rank | City | 2023 Population (Est.) | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Virginia Beach | 455,069 | -0.5% | 
| 2 | Chesapeake | 252,459 | 0.6% | 
| 3 | Norfolk | 232,558 | -1.1% | 
| 4 | Richmond | 228,367 | 0.4% | 
| 5 | Newport News | 183,980 | -0.5% | 
| 6 | Alexandria | 155,045 | -1.3% | 
| 7 | Hampton | 137,858 | 0.3% | 
| 8 | Suffolk | 98,426 | 2.0% | 
| 9 | Roanoke | 97,657 | -1.1% | 
| 10 | Portsmouth | 96,954 | -0.5% | 
| 11 | Lynchburg | 79,228 | 0.2% | 
| 12 | Harrisonburg | 51,150 | -0.5% | 
| 13 | Leesburg | 49,052 | 0.6% | 
| 14 | Blacksburg | 45,573 | 1.6% | 
| 15 | Charlottesville | 45,272 | -1.2% | 
| 16 | Manassas | 42,673 | -0.1% | 
| 17 | Danville | 42,017 | -0.5% | 
| 18 | Petersburg | 33,462 | 0.1% | 
| 19 | Fredericksburg | 28,648 | 1.2% | 
| 20 | Winchester | 27,861 | -0.3% | 
| 21 | Staunton | 25,940 | 0.3% | 
| 22 | Salem | 25,700 | 0.6% | 
| 23 | Fairfax | 24,752 | 1.3% | 
| 24 | Herndon | 24,186 | -0.9% | 
| 25 | Waynesboro | 22,799 | 1.2% | 
| 26 | Hopewell | 22,790 | -0.4% | 
| 27 | Christiansburg | 22,542 | -2.1% | 
| 28 | Culpeper | 20,750 | 1.5% | 
| 29 | Colonial Heights | 18,278 | 0.2% | 
| 30 | Bristol | 16,940 | -0.9% | 
| 31 | Radford | 16,719 | 1.9% | 
| 32 | Manassas Park | 16,693 | -1.3% | 
| 33 | Vienna | 16,291 | -0.5% | 
| 34 | Williamsburg | 15,685 | 0.8% | 
| 35 | Front Royal | 15,246 | 0.5% | 
| 36 | Falls Church | 14,489 | -0.6% | 
| 37 | Martinsville | 13,697 | 1.0% | 
| 38 | Poquoson | 12,624 | 0.7% | 
| 39 | Warrenton | 10,201 | 1.0% | 
| 40 | Purcellville | 8,990 | 0.1% | 
| 41 | Pulaski | 8,897 | -0.5% | 
| 42 | Smithfield | 8,866 | 1.8% | 
| 43 | Abingdon | 8,322 | -0.3% | 
| 44 | Franklin | 8,274 | 0.6% | 
| 45 | Wytheville | 8,182 | -0.6% | 
| 46 | Vinton | 8,030 | -0.1% | 
| 47 | South Boston | 7,854 | -0.8% | 
| 48 | Ashland | 7,748 | 1.7% | 
| 49 | Lexington | 7,506 | 1.3% | 
| 50 | Farmville | 7,466 | 1.9% | 
| 51 | Strasburg | 7,214 | 0.7% | 
| 52 | Bedford | 6,726 | 0.9% | 
| 53 | Galax | 6,702 | 0.1% | 
| 54 | Bridgewater | 6,695 | 4.4% | 
| 55 | Buena Vista | 6,569 | -0.4% | 
| 56 | Woodstock | 5,903 | 0.9% | 
| 57 | Dumfries | 5,757 | 0.4% | 
| 58 | Marion | 5,658 | -1.3% | 
| 59 | Covington | 5,633 | -0.8% | 
| 60 | Emporia | 5,533 | -1.6% | 
| 61 | Richlands | 5,157 | -1.1% | 
| 62 | Big Stone Gap | 5,141 | -1.1% | 
| 63 | Orange | 5,097 | 0.3% | 
| 64 | Bluefield | 4,970 | -1.1% | 
| 65 | Rocky Mount | 4,938 | 0.7% | 
| 66 | Luray | 4,839 | 0.7% | 
| 67 | South Hill | 4,755 | 0.8% | 
| 68 | Berryville | 4,743 | 2.3% | 
| 69 | Tazewell | 4,384 | -1.0% | 
| 70 | Broadway | 4,257 | 0.7% | 
| 71 | Colonial Beach | 3,956 | 0.8% | 
| 72 | Norton | 3,589 | -1.2% | 
| 73 | West Point | 3,443 | 0.4% | 
| 74 | Clifton Forge | 3,421 | -1.5% | 
| 75 | Blackstone | 3,335 | -0.4% | 
| 76 | Altavista | 3,326 | -0.8% | 
| 77 | Chincoteague | 3,287 | -0.6% | 
| 78 | Lebanon | 3,106 | -0.6% | 
| 79 | Timberville | 3,023 | 0.8% | 
| 80 | Elkton | 2,995 | 0.4% | 
| 81 | Grottoes | 2,955 | 0.6% | 
| 82 | Wise | 2,907 | -0.9% | 
| 83 | Hillsville | 2,858 | -0.7% | 
| 84 | Windsor | 2,856 | 1.4% | 
| 85 | Pearisburg | 2,828 | -1.4% | 
| 86 | Lovettsville | 2,728 | 0.4% | 
| 87 | Dublin | 2,669 | 0.5% | 
| 88 | Shenandoah | 2,486 | -0.0% | 
| 89 | Crewe | 2,238 | -0.7% | 
| 90 | Tappahannock | 2,195 | -1.1% | 
| 91 | New Market | 2,180 | 1.0% | 
| 92 | Amherst | 2,118 | -0.8% | 
| 93 | Louisa | 2,100 | 2.1% | 
| 94 | Stephens City | 2,091 | 1.6% | 
| 95 | Chase City | 2,056 | -0.0% | 
| 96 | Narrows | 2,046 | -1.0% | 
| 97 | Gate City | 2,030 | -0.4% | 
| 98 | Mount Jackson | 1,995 | 0.8% | 
| 99 | Appomattox | 1,977 | 2.2% | 
| 100 | Waverly | 1,915 | 1.1% | 
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