| Rank | City | 2009 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Louisville | 563,119 | 0.2% |
| 2 | Lexington | 292,240 | 1.4% |
| 3 | Bowling Green | 55,636 | 1.5% |
| 4 | Owensboro | 55,511 | 0.3% |
| 5 | Covington | 42,925 | -0.0% |
| 6 | Richmond | 32,943 | 2.2% |
| 7 | Hopkinsville | 32,046 | 0.8% |
| 8 | Florence | 27,891 | 2.0% |
| 9 | Henderson | 27,881 | 0.2% |
| 10 | Frankfort | 27,386 | -0.2% |
| 11 | Nicholasville | 26,580 | 3.1% |
| 12 | Jeffersontown | 26,307 | -0.1% |
| 13 | Paducah | 25,559 | -0.3% |
| 14 | Elizabethtown | 24,124 | 0.8% |
| 15 | Radcliff | 22,107 | 0.0% |
| 16 | Georgetown | 21,633 | 2.0% |
| 17 | Independence | 21,602 | 4.5% |
| 18 | Ashland | 21,318 | -0.3% |
| 19 | Madisonville | 19,052 | -0.2% |
| 20 | St. Matthews | 18,673 | 1.0% |
| 21 | Erlanger | 17,205 | 0.4% |
| 22 | Winchester | 16,622 | -0.1% |
| 23 | Murray | 16,470 | 1.0% |
| 24 | Shively | 16,389 | 1.2% |
| 25 | Newport | 15,759 | -1.0% |
| 26 | Danville | 15,621 | 0.1% |
| 27 | Fort Thomas | 15,212 | -1.0% |
| 28 | Berea | 14,511 | 3.1% |
| 29 | Glasgow | 14,190 | 1.0% |
| 30 | Somerset | 12,184 | 0.8% |
| 31 | Mount Washington | 11,973 | 4.1% |
| 32 | Shelbyville | 11,342 | 1.3% |
| 33 | Bardstown | 11,232 | 0.5% |
| 34 | Campbellsville | 11,200 | 0.9% |
| 35 | Lyndon | 11,155 | 1.3% |
| 36 | Mayfield | 10,156 | -0.2% |
| 37 | Lawrenceburg | 9,994 | 1.1% |
| 38 | Middlesborough | 9,867 | -0.6% |
| 39 | Shepherdsville | 9,246 | 1.0% |
| 40 | Paris | 9,223 | 0.0% |
| 41 | Maysville | 9,196 | 0.3% |
| 42 | Oak Grove | 9,162 | 3.2% |
| 43 | Edgewood | 8,917 | -0.6% |
| 44 | Alexandria | 8,397 | -0.2% |
| 45 | Corbin | 8,245 | 0.6% |
| 46 | Harrodsburg | 8,152 | -0.0% |
| 47 | Franklin | 7,993 | -0.2% |
| 48 | Elsmere | 7,981 | -0.3% |
| 49 | Versailles | 7,891 | -0.0% |
| 50 | London | 7,866 | 0.7% |
| 51 | Villa Hills | 7,718 | -0.3% |
| 52 | Morehead | 7,659 | 0.1% |
| 53 | Flatwoods | 7,621 | 0.2% |
| 54 | Fort Mitchell | 7,578 | -0.8% |
| 55 | Hillview | 7,479 | 0.8% |
| 56 | Russellville | 7,227 | 0.1% |
| 57 | Taylor Mill | 7,132 | -0.3% |
| 58 | Middletown | 7,121 | 2.5% |
| 59 | Mount Sterling | 6,941 | 1.6% |
| 60 | Highland Heights | 6,921 | 0.2% |
| 61 | La Grange | 6,556 | 1.7% |
| 62 | Leitchfield | 6,508 | 0.5% |
| 63 | Pikeville | 6,393 | -0.2% |
| 64 | Princeton | 6,393 | -0.3% |
| 65 | Lebanon | 6,332 | 0.9% |
| 66 | Cynthiana | 6,282 | -0.1% |
| 67 | Monticello | 6,129 | 0.3% |
| 68 | Douglass Hills | 6,079 | 1.1% |
| 69 | Wilmore | 5,954 | 0.1% |
| 70 | Bellevue | 5,854 | -1.2% |
| 71 | Cold Spring | 5,804 | 5.0% |
| 72 | Prospect | 5,792 | 2.8% |
| 73 | Central City | 5,682 | -0.4% |
| 74 | Dayton | 5,453 | -1.1% |
| 75 | Fort Wright | 5,452 | -0.5% |
| 76 | Williamsburg | 5,141 | 0.5% |
| 77 | Ludlow | 4,816 | 1.1% |
| 78 | Hazard | 4,757 | -0.3% |
| 79 | Columbia | 4,558 | 0.4% |
| 80 | Scottsville | 4,549 | 0.6% |
| 81 | Hurstbourne | 4,409 | 1.6% |
| 82 | Lancaster | 4,391 | 1.7% |
| 83 | Benton | 4,358 | 0.0% |
| 84 | Vine Grove | 4,353 | 0.6% |
| 85 | Greenville | 4,305 | -0.2% |
| 86 | Paintsville | 4,152 | 0.1% |
| 87 | Crescent Springs | 4,064 | 0.4% |
| 88 | Grayson | 3,908 | 0.1% |
| 89 | Carrollton | 3,905 | 0.2% |
| 90 | Prestonsburg | 3,830 | -0.1% |
| 91 | Crestview Hills | 3,697 | 3.0% |
| 92 | Union | 3,636 | 2.8% |
| 93 | Russell | 3,605 | -0.1% |
| 94 | Barbourville | 3,548 | -0.1% |
| 95 | Williamstown | 3,514 | 1.0% |
| 96 | Indian Hills | 3,470 | 2.3% |
| 97 | Providence | 3,409 | -0.7% |
| 98 | Stanford | 3,373 | -0.2% |
| 99 | West Liberty | 3,367 | 0.4% |
| 100 | Southgate | 3,279 | -0.7% |
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