| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Louisville | 553,960 | – |
| 2 | Lexington | 261,189 | 1.5% |
| 3 | Owensboro | 54,238 | 0.1% |
| 4 | Bowling Green | 49,528 | 1.6% |
| 5 | Covington | 42,986 | -0.1% |
| 6 | Hopkinsville | 30,176 | 0.0% |
| 7 | Frankfort | 27,775 | 0.3% |
| 8 | Richmond | 27,714 | 2.6% |
| 9 | Henderson | 27,451 | 0.5% |
| 10 | Jeffersontown | 26,518 | 1.4% |
| 11 | Paducah | 26,269 | -0.4% |
| 12 | Florence | 23,727 | 2.1% |
| 13 | Elizabethtown | 22,614 | 2.0% |
| 14 | Radcliff | 22,083 | 1.0% |
| 15 | Ashland | 21,907 | -0.8% |
| 16 | Nicholasville | 20,842 | 4.0% |
| 17 | Madisonville | 19,319 | -0.0% |
| 18 | Georgetown | 18,528 | 4.7% |
| 19 | St. Matthews | 17,310 | 1.0% |
| 20 | Newport | 17,029 | -1.0% |
| 21 | Winchester | 16,736 | 0.6% |
| 22 | Erlanger | 16,693 | 0.4% |
| 23 | Fort Thomas | 16,479 | 0.2% |
| 24 | Danville | 15,478 | 0.7% |
| 25 | Murray | 15,251 | 0.4% |
| 26 | Independence | 15,180 | 3.7% |
| 27 | Shively | 14,899 | -0.4% |
| 28 | Glasgow | 13,114 | 0.2% |
| 29 | Somerset | 11,413 | 0.3% |
| 30 | Berea | 11,328 | 2.0% |
| 31 | Bardstown | 10,803 | 3.0% |
| 32 | Campbellsville | 10,466 | 0.5% |
| 33 | Middlesborough | 10,371 | -0.9% |
| 34 | Mayfield | 10,357 | 0.0% |
| 35 | Shelbyville | 10,201 | 4.7% |
| 36 | Lyndon | 10,099 | 2.5% |
| 37 | Edgewood | 9,389 | 1.5% |
| 38 | Paris | 9,190 | 0.3% |
| 39 | Lawrenceburg | 9,163 | 3.7% |
| 40 | Maysville | 8,974 | -0.2% |
| 41 | Mount Washington | 8,711 | 4.8% |
| 42 | Alexandria | 8,542 | 3.6% |
| 43 | Shepherdsville | 8,512 | 5.8% |
| 44 | Harrodsburg | 8,158 | 1.0% |
| 45 | Elsmere | 8,157 | 1.8% |
| 46 | Franklin | 8,143 | 0.6% |
| 47 | Fort Mitchell | 8,074 | 0.6% |
| 48 | Villa Hills | 7,933 | 0.6% |
| 49 | Versailles | 7,908 | 0.9% |
| 50 | Corbin | 7,884 | 0.1% |
| 51 | Morehead | 7,603 | -1.1% |
| 52 | Flatwoods | 7,505 | -0.7% |
| 53 | London | 7,449 | 2.3% |
| 54 | Taylor Mill | 7,329 | 3.0% |
| 55 | Russellville | 7,152 | -0.5% |
| 56 | Oak Grove | 7,095 | 9.6% |
| 57 | Hillview | 7,039 | 1.3% |
| 58 | Highland Heights | 6,821 | 4.0% |
| 59 | Princeton | 6,525 | -0.8% |
| 60 | Pikeville | 6,485 | 0.1% |
| 61 | Bellevue | 6,467 | -0.8% |
| 62 | Cynthiana | 6,342 | -0.7% |
| 63 | Leitchfield | 6,233 | 1.4% |
| 64 | Mount Sterling | 6,130 | 1.1% |
| 65 | Monticello | 5,986 | 0.6% |
| 66 | Dayton | 5,956 | -0.8% |
| 67 | Wilmore | 5,899 | 3.4% |
| 68 | Lebanon | 5,899 | 0.3% |
| 69 | Central City | 5,883 | 1.5% |
| 70 | Middletown | 5,835 | 1.4% |
| 71 | La Grange | 5,713 | 3.6% |
| 72 | Fort Wright | 5,681 | -1.0% |
| 73 | Douglass Hills | 5,551 | 0.2% |
| 74 | Williamsburg | 4,948 | -1.1% |
| 75 | Hazard | 4,861 | -0.8% |
| 76 | Prospect | 4,626 | 5.0% |
| 77 | Columbia | 4,411 | 1.2% |
| 78 | Ludlow | 4,403 | -0.7% |
| 79 | Greenville | 4,386 | -1.2% |
| 80 | Benton | 4,346 | 0.8% |
| 81 | Scottsville | 4,325 | 0.2% |
| 82 | Vine Grove | 4,164 | 1.3% |
| 83 | Paintsville | 4,123 | -0.6% |
| 84 | Crescent Springs | 3,948 | 3.6% |
| 85 | Cold Spring | 3,941 | 1.7% |
| 86 | Grayson | 3,883 | 0.3% |
| 87 | Hurstbourne | 3,879 | -1.3% |
| 88 | Prestonsburg | 3,862 | 0.1% |
| 89 | Carrollton | 3,841 | 0.1% |
| 90 | Lancaster | 3,831 | 1.0% |
| 91 | Russell | 3,634 | -1.0% |
| 92 | Providence | 3,605 | -1.7% |
| 93 | Barbourville | 3,586 | -0.4% |
| 94 | Morganfield | 3,479 | -1.1% |
| 95 | Southgate | 3,472 | 1.1% |
| 96 | Stanford | 3,427 | 0.2% |
| 97 | Williamstown | 3,254 | 0.1% |
| 98 | West Liberty | 3,249 | 5.3% |
| 99 | Marion | 3,203 | -1.1% |
| 100 | Beaver Dam | 3,077 | 0.2% |
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