| Rank | City | 2006 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Louisville | 559,855 | 0.2% |
| 2 | Lexington | 278,313 | 1.3% |
| 3 | Owensboro | 55,146 | 0.3% |
| 4 | Bowling Green | 53,043 | 1.4% |
| 5 | Covington | 42,364 | -0.3% |
| 6 | Richmond | 31,612 | 2.7% |
| 7 | Hopkinsville | 31,329 | 0.8% |
| 8 | Henderson | 27,428 | -0.0% |
| 9 | Frankfort | 27,294 | -0.3% |
| 10 | Florence | 26,168 | 2.0% |
| 11 | Jeffersontown | 26,138 | -0.3% |
| 12 | Paducah | 25,441 | -0.6% |
| 13 | Nicholasville | 24,367 | 3.2% |
| 14 | Elizabethtown | 23,402 | 0.7% |
| 15 | Radcliff | 21,549 | -0.5% |
| 16 | Ashland | 21,241 | -0.6% |
| 17 | Georgetown | 20,143 | 1.7% |
| 18 | Madisonville | 19,032 | -0.3% |
| 19 | Independence | 19,013 | 4.6% |
| 20 | St. Matthews | 17,303 | -0.0% |
| 21 | Erlanger | 16,823 | 0.2% |
| 22 | Winchester | 16,406 | -0.4% |
| 23 | Murray | 15,888 | 0.8% |
| 24 | Newport | 15,878 | -1.4% |
| 25 | Fort Thomas | 15,541 | -1.2% |
| 26 | Danville | 15,418 | -0.1% |
| 27 | Shively | 14,974 | 0.1% |
| 28 | Glasgow | 14,055 | 1.4% |
| 29 | Berea | 13,303 | 3.3% |
| 30 | Somerset | 11,927 | 0.9% |
| 31 | Mount Washington | 11,604 | 5.9% |
| 32 | Campbellsville | 10,983 | 1.0% |
| 33 | Bardstown | 10,921 | 0.2% |
| 34 | Shelbyville | 10,737 | 1.0% |
| 35 | Mayfield | 10,186 | -0.3% |
| 36 | Lyndon | 10,179 | 0.2% |
| 37 | Middlesborough | 9,983 | -0.8% |
| 38 | Lawrenceburg | 9,379 | 0.5% |
| 39 | Paris | 9,263 | 0.2% |
| 40 | Shepherdsville | 8,971 | 1.1% |
| 41 | Maysville | 8,969 | -0.0% |
| 42 | Edgewood | 8,894 | -1.1% |
| 43 | Oak Grove | 8,745 | 4.3% |
| 44 | Alexandria | 8,184 | -0.9% |
| 45 | Harrodsburg | 8,170 | 0.0% |
| 46 | Corbin | 8,066 | 0.5% |
| 47 | Franklin | 8,046 | -0.2% |
| 48 | Elsmere | 7,959 | -0.5% |
| 49 | Versailles | 7,827 | -0.2% |
| 50 | Villa Hills | 7,719 | -0.5% |
| 51 | London | 7,712 | 0.7% |
| 52 | Fort Mitchell | 7,592 | -1.2% |
| 53 | Morehead | 7,572 | -0.1% |
| 54 | Flatwoods | 7,474 | -0.1% |
| 55 | Hillview | 7,296 | 0.7% |
| 56 | Russellville | 7,171 | 0.1% |
| 57 | Taylor Mill | 7,102 | -0.6% |
| 58 | Highland Heights | 6,826 | 0.0% |
| 59 | Mount Sterling | 6,488 | 1.1% |
| 60 | Leitchfield | 6,418 | 0.6% |
| 61 | Princeton | 6,341 | -0.6% |
| 62 | Pikeville | 6,299 | -0.6% |
| 63 | Lebanon | 6,256 | 1.2% |
| 64 | Cynthiana | 6,206 | -0.4% |
| 65 | La Grange | 6,179 | 1.6% |
| 66 | Monticello | 6,063 | 0.3% |
| 67 | Middletown | 6,050 | 0.7% |
| 68 | Bellevue | 6,006 | -1.5% |
| 69 | Central City | 5,735 | -0.5% |
| 70 | Wilmore | 5,700 | -0.7% |
| 71 | Douglass Hills | 5,587 | 0.1% |
| 72 | Dayton | 5,540 | -1.4% |
| 73 | Fort Wright | 5,429 | -0.9% |
| 74 | Cold Spring | 5,261 | 5.9% |
| 75 | Williamsburg | 5,075 | 0.5% |
| 76 | Prospect | 4,838 | 0.9% |
| 77 | Hazard | 4,753 | -0.4% |
| 78 | Ludlow | 4,641 | 1.1% |
| 79 | Columbia | 4,544 | 0.6% |
| 80 | Scottsville | 4,444 | 0.5% |
| 81 | Greenville | 4,316 | -0.3% |
| 82 | Benton | 4,312 | -0.2% |
| 83 | Lancaster | 4,226 | 2.0% |
| 84 | Paintsville | 4,195 | 0.3% |
| 85 | Crescent Springs | 3,983 | 0.2% |
| 86 | Vine Grove | 3,973 | -0.9% |
| 87 | Grayson | 3,969 | 0.4% |
| 88 | Hurstbourne | 3,941 | 0.3% |
| 89 | Prestonsburg | 3,841 | -0.1% |
| 90 | Carrollton | 3,836 | -0.0% |
| 91 | Russell | 3,564 | -0.4% |
| 92 | Providence | 3,506 | -0.6% |
| 93 | Barbourville | 3,506 | -0.5% |
| 94 | Stanford | 3,414 | -0.1% |
| 95 | Williamstown | 3,397 | 0.9% |
| 96 | Morganfield | 3,367 | -0.7% |
| 97 | West Liberty | 3,364 | 0.7% |
| 98 | Union | 3,359 | 2.8% |
| 99 | Crestview Hills | 3,351 | 2.8% |
| 100 | Southgate | 3,342 | -0.8% |
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