| Rank | City | 2005 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Louisville | 558,389 | 0.2% |
| 2 | Lexington | 274,581 | 1.3% |
| 3 | Owensboro | 54,786 | 0.3% |
| 4 | Bowling Green | 51,832 | 1.1% |
| 5 | Covington | 42,613 | -0.2% |
| 6 | Hopkinsville | 31,111 | 0.8% |
| 7 | Richmond | 30,403 | 2.3% |
| 8 | Henderson | 27,392 | -0.1% |
| 9 | Frankfort | 27,382 | -0.4% |
| 10 | Jeffersontown | 26,281 | -0.2% |
| 11 | Florence | 25,425 | 1.7% |
| 12 | Paducah | 25,350 | -0.9% |
| 13 | Nicholasville | 23,408 | 2.9% |
| 14 | Elizabethtown | 23,076 | 0.5% |
| 15 | Radcliff | 21,603 | -0.5% |
| 16 | Ashland | 21,404 | -0.6% |
| 17 | Georgetown | 19,813 | 1.7% |
| 18 | Madisonville | 19,147 | -0.2% |
| 19 | Independence | 17,928 | 4.2% |
| 20 | St. Matthews | 17,332 | 0.0% |
| 21 | Erlanger | 16,743 | 0.1% |
| 22 | Winchester | 16,374 | -0.5% |
| 23 | Newport | 16,067 | -1.4% |
| 24 | Fort Thomas | 15,698 | -1.2% |
| 25 | Murray | 15,657 | 0.7% |
| 26 | Danville | 15,398 | -0.1% |
| 27 | Shively | 14,985 | 0.1% |
| 28 | Glasgow | 13,816 | 1.3% |
| 29 | Berea | 12,768 | 3.0% |
| 30 | Somerset | 11,798 | 0.8% |
| 31 | Bardstown | 10,860 | 0.1% |
| 32 | Campbellsville | 10,811 | 0.8% |
| 33 | Mount Washington | 10,639 | 5.1% |
| 34 | Shelbyville | 10,587 | 0.9% |
| 35 | Lyndon | 10,180 | 0.2% |
| 36 | Mayfield | 10,159 | -0.5% |
| 37 | Middlesborough | 10,065 | -0.7% |
| 38 | Lawrenceburg | 9,361 | 0.5% |
| 39 | Paris | 9,257 | 0.2% |
| 40 | Edgewood | 9,007 | -1.0% |
| 41 | Shepherdsville | 8,908 | 1.1% |
| 42 | Maysville | 8,899 | -0.2% |
| 43 | Oak Grove | 8,666 | 5.1% |
| 44 | Alexandria | 8,237 | -0.9% |
| 45 | Harrodsburg | 8,149 | -0.0% |
| 46 | Elsmere | 8,072 | -0.3% |
| 47 | Franklin | 8,028 | -0.4% |
| 48 | Corbin | 7,977 | 0.3% |
| 49 | Versailles | 7,819 | -0.3% |
| 50 | Villa Hills | 7,786 | -0.5% |
| 51 | Fort Mitchell | 7,691 | -1.2% |
| 52 | London | 7,669 | 0.7% |
| 53 | Morehead | 7,553 | -0.2% |
| 54 | Flatwoods | 7,490 | -0.1% |
| 55 | Hillview | 7,264 | 0.8% |
| 56 | Taylor Mill | 7,173 | -0.5% |
| 57 | Russellville | 7,140 | -0.0% |
| 58 | Highland Heights | 6,872 | 0.2% |
| 59 | Leitchfield | 6,377 | 0.6% |
| 60 | Princeton | 6,339 | -0.7% |
| 61 | Pikeville | 6,328 | -0.6% |
| 62 | Mount Sterling | 6,322 | 0.8% |
| 63 | Cynthiana | 6,223 | -0.5% |
| 64 | Lebanon | 6,219 | 1.3% |
| 65 | Bellevue | 6,080 | -1.5% |
| 66 | Monticello | 6,064 | 0.3% |
| 67 | La Grange | 6,044 | 1.4% |
| 68 | Middletown | 6,018 | 0.8% |
| 69 | Central City | 5,754 | -0.6% |
| 70 | Wilmore | 5,719 | -0.8% |
| 71 | Dayton | 5,609 | -1.5% |
| 72 | Douglass Hills | 5,589 | 0.2% |
| 73 | Fort Wright | 5,494 | -0.8% |
| 74 | Williamsburg | 5,047 | 0.5% |
| 75 | Cold Spring | 5,002 | 6.1% |
| 76 | Prospect | 4,803 | 0.9% |
| 77 | Hazard | 4,764 | -0.5% |
| 78 | Columbia | 4,507 | 0.5% |
| 79 | Ludlow | 4,421 | 0.1% |
| 80 | Scottsville | 4,404 | 0.5% |
| 81 | Greenville | 4,354 | -0.2% |
| 82 | Benton | 4,300 | -0.3% |
| 83 | Paintsville | 4,161 | 0.2% |
| 84 | Lancaster | 4,120 | 1.8% |
| 85 | Crescent Springs | 3,981 | 0.2% |
| 86 | Grayson | 3,966 | 0.5% |
| 87 | Vine Grove | 3,964 | -1.2% |
| 88 | Hurstbourne | 3,935 | 0.4% |
| 89 | Prestonsburg | 3,856 | -0.0% |
| 90 | Carrollton | 3,805 | -0.2% |
| 91 | Russell | 3,573 | -0.4% |
| 92 | Providence | 3,513 | -0.6% |
| 93 | Barbourville | 3,492 | -0.7% |
| 94 | Morganfield | 3,394 | -0.6% |
| 95 | West Liberty | 3,393 | 1.1% |
| 96 | Stanford | 3,389 | -0.3% |
| 97 | Williamstown | 3,387 | 1.0% |
| 98 | Southgate | 3,381 | -0.7% |
| 99 | Crestview Hills | 3,280 | 2.9% |
| 100 | Union | 3,240 | 2.6% |
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