| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Des Moines | 199,607 | 0.3% |
| 2 | Cedar Rapids | 121,836 | 1.1% |
| 3 | Davenport | 98,228 | 0.3% |
| 4 | Sioux City | 84,965 | 0.5% |
| 5 | Waterloo | 68,766 | 0.3% |
| 6 | Iowa City | 62,865 | 0.5% |
| 7 | Council Bluffs | 58,346 | 0.6% |
| 8 | Dubuque | 57,696 | -0.0% |
| 9 | Ames | 50,927 | 0.7% |
| 10 | West Des Moines | 47,182 | 3.9% |
| 11 | Cedar Falls | 36,202 | 0.7% |
| 12 | Bettendorf | 31,295 | 1.2% |
| 13 | Urbandale | 29,504 | 2.3% |
| 14 | Mason City | 29,167 | -0.0% |
| 15 | Clinton | 27,700 | -0.6% |
| 16 | Ankeny | 27,584 | 4.0% |
| 17 | Burlington | 26,775 | -0.2% |
| 18 | Marion | 26,567 | 2.6% |
| 19 | Fort Dodge | 26,297 | – |
| 20 | Marshalltown | 26,014 | 0.3% |
| 21 | Ottumwa | 24,952 | 0.1% |
| 22 | Muscatine | 22,700 | -0.1% |
| 23 | Newton | 15,605 | 0.5% |
| 24 | Coralville | 15,214 | 3.6% |
| 25 | Indianola | 13,189 | 1.7% |
| 26 | Clive | 12,995 | 5.6% |
| 27 | Boone | 12,810 | 0.3% |
| 28 | Fort Madison | 11,635 | -0.2% |
| 29 | Keokuk | 11,376 | -1.2% |
| 30 | Spencer | 11,337 | 0.1% |
| 31 | Oskaloosa | 10,987 | 0.2% |
| 32 | Altoona | 10,449 | 3.5% |
| 33 | Carroll | 10,067 | 0.4% |
| 34 | Storm Lake | 10,047 | 1.2% |
| 35 | Pella | 9,947 | 0.6% |
| 36 | Fairfield | 9,592 | -0.4% |
| 37 | Le Mars | 9,241 | 0.6% |
| 38 | Grinnell | 9,101 | 0.1% |
| 39 | Johnston | 8,995 | 6.2% |
| 40 | Waverly | 8,976 | 0.3% |
| 41 | Mount Pleasant | 8,728 | 0.8% |
| 42 | Clear Lake | 8,176 | -0.5% |
| 43 | Webster City | 8,168 | 0.2% |
| 44 | Decorah | 8,166 | 0.0% |
| 45 | Charles City | 7,790 | -0.2% |
| 46 | Knoxville | 7,740 | -0.7% |
| 47 | Perry | 7,678 | 1.4% |
| 48 | Creston | 7,570 | -0.5% |
| 49 | Denison | 7,339 | 1.0% |
| 50 | Atlantic | 7,254 | -0.4% |
| 51 | Washington | 7,062 | -0.1% |
| 52 | Norwalk | 7,055 | 2.1% |
| 53 | Nevada | 6,689 | 1.0% |
| 54 | Oelwein | 6,680 | -0.4% |
| 55 | Estherville | 6,620 | -0.2% |
| 56 | Hiawatha | 6,520 | 1.8% |
| 57 | Red Oak | 6,216 | -0.1% |
| 58 | Maquoketa | 6,138 | -0.2% |
| 59 | Sioux Center | 6,076 | 1.9% |
| 60 | Independence | 6,061 | -0.0% |
| 61 | Centerville | 5,909 | -0.3% |
| 62 | Algona | 5,735 | -0.8% |
| 63 | Clarinda | 5,673 | 1.0% |
| 64 | Orange City | 5,632 | 1.1% |
| 65 | Waukee | 5,582 | 7.0% |
| 66 | Shenandoah | 5,525 | -0.2% |
| 67 | Anamosa | 5,503 | 0.7% |
| 68 | North Liberty | 5,482 | 6.0% |
| 69 | Glenwood | 5,396 | 0.0% |
| 70 | Cherokee | 5,354 | -1.2% |
| 71 | Harlan | 5,286 | 0.1% |
| 72 | Manchester | 5,275 | 0.2% |
| 73 | Pleasant Hill | 5,250 | 3.2% |
| 74 | Iowa Falls | 5,194 | -0.6% |
| 75 | Grimes | 5,125 | 6.7% |
| 76 | Vinton | 5,106 | -0.0% |
| 77 | De Witt | 5,044 | 0.9% |
| 78 | Sheldon | 5,018 | 0.1% |
| 79 | Windsor Heights | 4,773 | -0.7% |
| 80 | Osceola | 4,769 | 1.3% |
| 81 | Winterset | 4,760 | 0.6% |
| 82 | Jefferson | 4,614 | 0.5% |
| 83 | Chariton | 4,585 | -0.2% |
| 84 | Evansdale | 4,523 | -0.3% |
| 85 | Humboldt | 4,458 | -0.3% |
| 86 | Forest City | 4,396 | -0.1% |
| 87 | Spirit Lake | 4,273 | 0.9% |
| 88 | Eldridge | 4,219 | 1.7% |
| 89 | Camanche | 4,210 | -0.6% |
| 90 | Hampton | 4,208 | 0.1% |
| 91 | Waukon | 4,128 | -0.9% |
| 92 | Dyersville | 4,050 | 0.9% |
| 93 | Emmetsburg | 3,901 | -0.2% |
| 94 | Cresco | 3,899 | 0.6% |
| 95 | Mount Vernon | 3,821 | 0.4% |
| 96 | Albia | 3,760 | -0.8% |
| 97 | Eagle Grove | 3,706 | 0.1% |
| 98 | New Hampton | 3,687 | -0.4% |
| 99 | Monticello | 3,648 | 0.3% |
| 100 | Carlisle | 3,589 | 0.9% |
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