| Rank | City | 1890 Population | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Des Moines | 50,093 | 8.4% | 
| 2 | Sioux City | 37,806 | – | 
In 1890, the largest city in Iowa was Des Moines, with a population of 50,093. The second largest city is Sioux City, with a population of 37,806.
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