| Rank | City | 2016 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Sioux Falls | 171,544 | 2.2% |
| 2 | Rapid City | 73,569 | 1.6% |
| 3 | Aberdeen | 28,102 | 1.5% |
| 4 | Brookings | 23,657 | 1.4% |
| 5 | Watertown | 22,073 | 0.5% |
| 6 | Mitchell | 15,669 | 0.5% |
| 7 | Yankton | 14,557 | 0.1% |
| 8 | Pierre | 14,002 | 0.5% |
| 9 | Huron | 13,313 | 1.1% |
| 10 | Spearfish | 11,283 | 1.5% |
| 11 | Vermillion | 10,738 | 0.3% |
| 12 | Brandon | 9,856 | 2.3% |
| 13 | Box Elder | 9,289 | 3.6% |
| 14 | Madison | 7,258 | 2.3% |
| 15 | Sturgis | 6,688 | 0.2% |
| 16 | Belle Fourche | 5,696 | 0.4% |
| 17 | Harrisburg | 5,498 | 6.1% |
| 18 | Tea | 4,839 | 4.9% |
| 19 | Dell Rapids | 3,706 | 0.4% |
| 20 | Hot Springs | 3,532 | -1.0% |
| 21 | Mobridge | 3,466 | 0.0% |
| 22 | Canton | 3,331 | 1.7% |
| 23 | Milbank | 3,192 | -1.0% |
| 24 | Hartford | 3,025 | 3.6% |
| 25 | Lead | 2,999 | -0.8% |
| 26 | Winner | 2,825 | -0.5% |
| 27 | North Sioux City | 2,731 | 1.5% |
| 28 | Sisseton | 2,450 | -0.2% |
| 29 | Chamberlain | 2,386 | -0.0% |
| 30 | Redfield | 2,379 | 0.4% |
| 31 | Flandreau | 2,303 | -0.3% |
| 32 | Lennox | 2,272 | 1.5% |
| 33 | Summerset | 2,239 | 4.3% |
| 34 | Fort Pierre | 2,117 | 0.4% |
| 35 | Beresford | 1,976 | -0.3% |
| 36 | Springfield | 1,953 | -0.4% |
| 37 | Custer | 1,952 | -1.1% |
| 38 | Elk Point | 1,874 | -0.9% |
| 39 | Volga | 1,865 | 1.1% |
| 40 | Webster | 1,802 | -0.9% |
| 41 | Wagner | 1,589 | 0.3% |
| 42 | Groton | 1,508 | 0.7% |
| 43 | Parkston | 1,488 | -0.3% |
| 44 | Miller | 1,437 | -0.7% |
| 45 | Eagle Butte | 1,349 | 0.5% |
| 46 | Crooks | 1,329 | 0.9% |
| 47 | Salem | 1,325 | -0.3% |
| 48 | Freeman | 1,287 | -0.3% |
| 49 | Platte | 1,266 | 0.6% |
| 50 | Deadwood | 1,258 | -0.2% |
| 51 | Gregory | 1,254 | -0.6% |
| 52 | Clear Lake | 1,254 | -0.3% |
| 53 | Britton | 1,242 | 0.0% |
| 54 | Lemmon | 1,232 | 0.1% |
| 55 | Mission | 1,215 | 0.6% |
| 56 | Garretson | 1,201 | 0.6% |
| 57 | Gettysburg | 1,164 | 0.0% |
| 58 | Baltic | 1,132 | 0.8% |
| 59 | De Smet | 1,072 | -0.3% |
| 60 | Martin | 1,061 | -0.2% |
| 61 | Clark | 1,050 | -1.6% |
| 62 | Tyndall | 1,049 | -0.3% |
| 63 | Hill City | 995 | 1.0% |
| 64 | Parker | 993 | -0.6% |
| 65 | Ipswich | 959 | 0.1% |
| 66 | Worthing | 942 | 1.4% |
| 67 | Whitewood | 919 | -0.2% |
| 68 | Wessington Springs | 919 | -0.8% |
| 69 | Arlington | 883 | -0.7% |
| 70 | Wall | 877 | 2.7% |
| 71 | Centerville | 868 | -0.3% |
| 72 | Eureka | 844 | -0.6% |
| 73 | Lake Andes | 840 | -0.9% |
| 74 | Piedmont | 827 | 30.1% |
| 75 | Scotland | 820 | -0.5% |
| 76 | Howard | 790 | -1.6% |
| 77 | Highmore | 779 | -0.4% |
| 78 | Valley Springs | 767 | 0.2% |
| 79 | Marion | 767 | -0.4% |
| 80 | Viborg | 762 | -0.5% |
| 81 | Alcester | 761 | -1.2% |
| 82 | Estelline | 756 | -0.3% |
| 83 | Philip | 751 | -0.7% |
| 84 | Faulkton | 740 | 0.1% |
| 85 | Edgemont | 739 | -0.9% |
| 86 | Elkton | 731 | -0.1% |
| 87 | Plankinton | 714 | 0.2% |
| 88 | Kadoka | 707 | 1.6% |
| 89 | Colton | 690 | 0.1% |
| 90 | Kimball | 686 | -0.5% |
| 91 | Armour | 686 | -0.4% |
| 92 | McLaughlin | 679 | 0.5% |
| 93 | New Underwood | 672 | 0.4% |
| 94 | Onida | 663 | 0.2% |
| 95 | Woonsocket | 659 | 0.1% |
| 96 | Aurora | 650 | 4.1% |
| 97 | Canistota | 638 | -0.6% |
| 98 | Castlewood | 636 | 0.3% |
| 99 | Selby | 634 | -0.3% |
| 100 | Tripp | 630 | -0.5% |
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