| Rank | City | 2013 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Sioux Falls | 159,908 | 1.9% |
| 2 | Rapid City | 69,854 | 1.4% |
| 3 | Aberdeen | 26,791 | 1.3% |
| 4 | Brookings | 22,591 | 1.2% |
| 5 | Watertown | 21,803 | 0.7% |
| 6 | Mitchell | 15,484 | 0.8% |
| 7 | Yankton | 14,538 | 0.3% |
| 8 | Pierre | 13,914 | 1.0% |
| 9 | Huron | 12,867 | 1.1% |
| 10 | Vermillion | 10,811 | 1.1% |
| 11 | Spearfish | 10,718 | 1.1% |
| 12 | Brandon | 9,286 | 2.8% |
| 13 | Box Elder | 8,753 | 5.9% |
| 14 | Madison | 6,809 | 2.6% |
| 15 | Sturgis | 6,644 | 0.1% |
| 16 | Belle Fourche | 5,658 | 0.6% |
| 17 | Harrisburg | 4,653 | 6.7% |
| 18 | Tea | 4,300 | 6.3% |
| 19 | Dell Rapids | 3,689 | 0.8% |
| 20 | Hot Springs | 3,570 | -1.9% |
| 21 | Mobridge | 3,476 | 0.2% |
| 22 | Milbank | 3,303 | -0.7% |
| 23 | Canton | 3,224 | 2.7% |
| 24 | Lead | 3,088 | -0.6% |
| 25 | Winner | 2,847 | -0.9% |
| 26 | Hartford | 2,810 | 5.3% |
| 27 | North Sioux City | 2,546 | 0.3% |
| 28 | Sisseton | 2,497 | 0.5% |
| 29 | Chamberlain | 2,403 | 0.3% |
| 30 | Redfield | 2,392 | 1.3% |
| 31 | Flandreau | 2,318 | -0.5% |
| 32 | Lennox | 2,201 | 2.1% |
| 33 | Fort Pierre | 2,111 | 0.8% |
| 34 | Beresford | 2,040 | 0.9% |
| 35 | Elk Point | 1,992 | 0.7% |
| 36 | Custer | 1,988 | -1.9% |
| 37 | Springfield | 1,981 | -0.2% |
| 38 | Summerset | 1,898 | 2.3% |
| 39 | Webster | 1,851 | -0.9% |
| 40 | Volga | 1,780 | 0.3% |
| 41 | Wagner | 1,573 | 0.2% |
| 42 | Groton | 1,483 | 0.9% |
| 43 | Parkston | 1,482 | -0.9% |
| 44 | Miller | 1,467 | -0.7% |
| 45 | Eagle Butte | 1,352 | 1.3% |
| 46 | Salem | 1,345 | -0.1% |
| 47 | Crooks | 1,307 | 1.5% |
| 48 | Gregory | 1,284 | -0.4% |
| 49 | Clear Lake | 1,273 | – |
| 50 | Freeman | 1,271 | -1.3% |
| 51 | Deadwood | 1,263 | -0.3% |
| 52 | Lemmon | 1,245 | 0.7% |
| 53 | Platte | 1,241 | 0.4% |
| 54 | Britton | 1,238 | -0.1% |
| 55 | Mission | 1,219 | 1.6% |
| 56 | Garretson | 1,207 | 1.7% |
| 57 | Gettysburg | 1,177 | 0.6% |
| 58 | Baltic | 1,129 | 1.8% |
| 59 | De Smet | 1,110 | 1.0% |
| 60 | Martin | 1,072 | 0.0% |
| 61 | Clark | 1,068 | -3.2% |
| 62 | Tyndall | 1,057 | -0.5% |
| 63 | Parker | 1,014 | -0.4% |
| 64 | Hill City | 966 | 0.9% |
| 65 | Ipswich | 954 | – |
| 66 | Wessington Springs | 946 | -0.5% |
| 67 | Whitewood | 934 | 0.4% |
| 68 | Arlington | 927 | 0.7% |
| 69 | Worthing | 915 | 2.1% |
| 70 | Centerville | 886 | 0.2% |
| 71 | Eureka | 856 | -0.7% |
| 72 | Wall | 843 | 4.9% |
| 73 | Scotland | 834 | -0.4% |
| 74 | Lake Andes | 828 | -2.9% |
| 75 | Howard | 824 | -2.0% |
| 76 | Alcester | 809 | 0.1% |
| 77 | Highmore | 804 | 0.6% |
| 78 | Philip | 780 | 0.1% |
| 79 | Viborg | 775 | -0.4% |
| 80 | Marion | 775 | -0.6% |
| 81 | Valley Springs | 768 | 0.6% |
| 82 | Estelline | 760 | -0.5% |
| 83 | Edgemont | 753 | -1.4% |
| 84 | Faulkton | 740 | 0.3% |
| 85 | Elkton | 738 | 0.1% |
| 86 | Plankinton | 719 | 0.8% |
| 87 | Kimball | 707 | 0.3% |
| 88 | Colton | 693 | 0.4% |
| 89 | Armour | 690 | -0.6% |
| 90 | Kadoka | 685 | 2.3% |
| 91 | Onida | 680 | 1.7% |
| 92 | New Underwood | 673 | 1.0% |
| 93 | McLaughlin | 667 | 0.3% |
| 94 | Canistota | 653 | -0.2% |
| 95 | Woonsocket | 646 | -0.7% |
| 96 | Selby | 641 | -0.1% |
| 97 | Tripp | 630 | -1.3% |
| 98 | Castlewood | 625 | -0.2% |
| 99 | Alexandria | 623 | 0.6% |
| 100 | Lake Preston | 605 | 0.5% |
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