| Rank | City | 2010 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Sioux Falls | 158,008 | 2.6% |
| 2 | Rapid City | 67,107 | 1.2% |
| 3 | Aberdeen | 24,992 | -0.1% |
| 4 | Watertown | 20,350 | 0.0% |
| 5 | Brookings | 20,184 | 0.8% |
| 6 | Mitchell | 14,747 | 0.1% |
| 7 | Pierre | 14,072 | 0.2% |
| 8 | Yankton | 13,866 | 0.3% |
| 9 | Huron | 11,281 | -0.6% |
| 10 | Vermillion | 10,417 | 0.2% |
| 11 | Spearfish | 9,979 | 1.6% |
| 12 | Brandon | 8,106 | 3.8% |
| 13 | Madison | 6,605 | 0.1% |
| 14 | Sturgis | 5,981 | -0.9% |
| 15 | Harrisburg | 5,410 | 20.8% |
| 16 | Belle Fourche | 4,985 | 0.4% |
| 17 | Tea | 4,623 | 11.1% |
| 18 | Box Elder | 4,271 | 2.5% |
| 19 | Canton | 4,269 | 3.5% |
| 20 | Hot Springs | 4,093 | -0.1% |
| 21 | Dell Rapids | 3,176 | 0.7% |
| 22 | Milbank | 3,167 | -1.5% |
| 23 | Mobridge | 3,142 | -1.3% |
| 24 | Lead | 2,848 | -0.7% |
| 25 | Lennox | 2,842 | 3.7% |
| 26 | Winner | 2,671 | -1.7% |
| 27 | North Sioux City | 2,601 | 1.4% |
| 28 | Sisseton | 2,454 | -0.4% |
| 29 | Chamberlain | 2,292 | -0.3% |
| 30 | Beresford | 2,279 | 1.4% |
| 31 | Hartford | 2,277 | 2.1% |
| 32 | Redfield | 2,232 | -1.4% |
| 33 | Flandreau | 2,201 | -0.8% |
| 34 | Fort Pierre | 2,103 | 0.3% |
| 35 | Elk Point | 1,997 | 1.7% |
| 36 | Custer | 1,980 | 0.1% |
| 37 | Webster | 1,661 | -1.7% |
| 38 | Volga | 1,606 | 1.1% |
| 39 | Wagner | 1,506 | -0.7% |
| 40 | Springfield | 1,474 | -0.6% |
| 41 | Parkston | 1,458 | -1.5% |
| 42 | Groton | 1,416 | 0.5% |
| 43 | Miller | 1,323 | -1.6% |
| 44 | Salem | 1,306 | -0.7% |
| 45 | Platte | 1,296 | -0.6% |
| 46 | Deadwood | 1,272 | -0.9% |
| 47 | Britton | 1,218 | -0.9% |
| 48 | Mission | 1,207 | 3.2% |
| 49 | Clear Lake | 1,192 | -1.2% |
| 50 | Freeman | 1,177 | -1.2% |
| 51 | Worthing | 1,174 | 7.7% |
| 52 | Lemmon | 1,169 | -1.9% |
| 53 | Gregory | 1,158 | -2.0% |
| 54 | Crooks | 1,118 | 2.9% |
| 55 | Garretson | 1,113 | -0.5% |
| 56 | Tyndall | 1,082 | -1.5% |
| 57 | De Smet | 1,074 | -1.3% |
| 58 | Clark | 1,044 | -2.2% |
| 59 | Gettysburg | 1,020 | -2.9% |
| 60 | Martin | 981 | -1.0% |
| 61 | Parker | 971 | -0.8% |
| 62 | Eagle Butte | 932 | 0.3% |
| 63 | Baltic | 930 | 1.4% |
| 64 | Hill City | 927 | 1.9% |
| 65 | Eureka | 926 | -1.9% |
| 66 | Arlington | 920 | -0.8% |
| 67 | Alcester | 915 | 0.4% |
| 68 | Howard | 885 | -2.1% |
| 69 | Whitewood | 856 | 0.2% |
| 70 | Ipswich | 844 | -1.2% |
| 71 | Wessington Springs | 831 | -2.1% |
| 72 | Wall | 821 | 0.0% |
| 73 | Marion | 811 | -1.0% |
| 74 | Edgemont | 792 | -0.9% |
| 75 | Scotland | 777 | -1.5% |
| 76 | St. Francis | 761 | 0.9% |
| 77 | Lake Andes | 760 | -0.8% |
| 78 | Viborg | 759 | -1.0% |
| 79 | McLaughlin | 722 | -0.7% |
| 80 | Philip | 714 | -2.3% |
| 81 | Kimball | 700 | -0.6% |
| 82 | New Underwood | 690 | 1.1% |
| 83 | Estelline | 668 | -0.2% |
| 84 | Elkton | 655 | -0.4% |
| 85 | Faulkton | 654 | -2.0% |
| 86 | Armour | 652 | -1.9% |
| 87 | Canistota | 647 | -0.9% |
| 88 | Selby | 647 | -1.4% |
| 89 | Menno | 644 | -1.3% |
| 90 | Lake Preston | 643 | -1.5% |
| 91 | Alexandria | 641 | 1.4% |
| 92 | Woonsocket | 634 | -1.4% |
| 93 | Presho | 633 | 0.7% |
| 94 | Kadoka | 623 | -1.4% |
| 95 | Newell | 622 | -0.5% |
| 96 | Colton | 620 | -0.7% |
| 97 | Tripp | 619 | -1.5% |
| 98 | Jefferson | 618 | 0.6% |
| 99 | Onida | 615 | -2.0% |
| 100 | Waubay | 589 | -1.2% |
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