| Rank | City | 2005 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Sioux Falls | 141,488 | 3.0% |
| 2 | Rapid City | 61,950 | 0.8% |
| 3 | Aberdeen | 24,767 | -0.4% |
| 4 | Watertown | 20,151 | -0.1% |
| 5 | Brookings | 19,016 | 0.3% |
| 6 | Mitchell | 14,766 | 0.3% |
| 7 | Pierre | 13,900 | 0.0% |
| 8 | Yankton | 13,689 | 0.3% |
| 9 | Huron | 11,107 | -1.6% |
| 10 | Vermillion | 10,115 | -0.3% |
| 11 | Spearfish | 9,261 | 1.6% |
| 12 | Brandon | 6,498 | 2.8% |
| 13 | Sturgis | 6,344 | -0.5% |
| 14 | Madison | 6,185 | -1.3% |
| 15 | Belle Fourche | 4,790 | -0.1% |
| 16 | Hot Springs | 4,047 | -0.6% |
| 17 | Canton | 3,601 | 3.5% |
| 18 | Box Elder | 3,438 | 0.1% |
| 19 | Milbank | 3,400 | -1.6% |
| 20 | Mobridge | 3,270 | -2.0% |
| 21 | Dell Rapids | 3,013 | 0.1% |
| 22 | Lead | 2,971 | -0.4% |
| 23 | Winner | 2,913 | -1.7% |
| 24 | Tea | 2,658 | 10.4% |
| 25 | Sisseton | 2,520 | -0.2% |
| 26 | North Sioux City | 2,458 | 1.8% |
| 27 | Lennox | 2,370 | 3.6% |
| 28 | Redfield | 2,328 | -2.0% |
| 29 | Flandreau | 2,284 | -0.9% |
| 30 | Chamberlain | 2,246 | -1.1% |
| 31 | Fort Pierre | 2,077 | 0.4% |
| 32 | Custer | 2,042 | 0.9% |
| 33 | Beresford | 2,025 | 0.3% |
| 34 | Hartford | 1,915 | 0.4% |
| 35 | Elk Point | 1,812 | 1.4% |
| 36 | Webster | 1,786 | -2.1% |
| 37 | Harrisburg | 1,739 | 15.2% |
| 38 | Parkston | 1,556 | -1.7% |
| 39 | Wagner | 1,520 | -1.4% |
| 40 | Springfield | 1,517 | -0.7% |
| 41 | Volga | 1,478 | 0.4% |
| 42 | Miller | 1,383 | -2.5% |
| 43 | Salem | 1,378 | -0.2% |
| 44 | Groton | 1,355 | 0.0% |
| 45 | Deadwood | 1,325 | -0.9% |
| 46 | Platte | 1,296 | -1.3% |
| 47 | Britton | 1,252 | -1.4% |
| 48 | Gregory | 1,249 | -2.7% |
| 49 | Clear Lake | 1,241 | -1.7% |
| 50 | Lemmon | 1,235 | -2.9% |
| 51 | Freeman | 1,220 | -1.8% |
| 52 | Gettysburg | 1,199 | -2.6% |
| 53 | Clark | 1,164 | -2.3% |
| 54 | Tyndall | 1,148 | -1.8% |
| 55 | De Smet | 1,104 | -2.1% |
| 56 | Garretson | 1,099 | -1.5% |
| 57 | Martin | 1,025 | -1.2% |
| 58 | Parker | 1,005 | -0.9% |
| 59 | Eureka | 1,005 | -2.3% |
| 60 | Crooks | 959 | 2.6% |
| 61 | Howard | 947 | -3.0% |
| 62 | Eagle Butte | 944 | 0.9% |
| 63 | Mission | 941 | 1.0% |
| 64 | Arlington | 933 | -1.5% |
| 65 | Wessington Springs | 908 | -2.5% |
| 66 | Alcester | 882 | 0.1% |
| 67 | Ipswich | 874 | -1.8% |
| 68 | Baltic | 874 | 1.5% |
| 69 | Centerville | 867 | -1.1% |
| 70 | Whitewood | 850 | 0.2% |
| 71 | Hill City | 842 | 1.8% |
| 72 | Marion | 838 | -1.5% |
| 73 | Worthing | 824 | 8.3% |
| 74 | Scotland | 823 | -1.9% |
| 75 | Wall | 822 | 0.1% |
| 76 | Edgemont | 814 | -1.3% |
| 77 | Viborg | 802 | -1.0% |
| 78 | Highmore | 798 | -1.5% |
| 79 | Philip | 784 | -2.8% |
| 80 | Valley Springs | 775 | -0.6% |
| 81 | Lake Andes | 771 | -1.4% |
| 82 | McLaughlin | 763 | -0.1% |
| 83 | St. Francis | 753 | 1.7% |
| 84 | Armour | 728 | -1.6% |
| 85 | Faulkton | 704 | -2.6% |
| 86 | Canistota | 692 | -0.4% |
| 87 | Menno | 691 | -1.1% |
| 88 | Kimball | 691 | -1.8% |
| 89 | Woonsocket | 689 | -1.1% |
| 90 | Selby | 684 | -1.7% |
| 91 | Estelline | 671 | -0.3% |
| 92 | Tripp | 670 | -1.5% |
| 93 | Lake Preston | 667 | -2.5% |
| 94 | Castlewood | 663 | -0.2% |
| 95 | New Underwood | 656 | 1.3% |
| 96 | Onida | 654 | -2.9% |
| 97 | Alexandria | 652 | 3.6% |
| 98 | Kadoka | 652 | -2.0% |
| 99 | Elkton | 640 | -1.4% |
| 100 | Colton | 634 | -1.1% |
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