| Rank | City | 2016 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Des Moines | 210,330 | 0.7% |
| 2 | Cedar Rapids | 130,405 | 0.6% |
| 3 | Davenport | 102,582 | 0.6% |
| 4 | Sioux City | 82,821 | 0.0% |
| 5 | Iowa City | 74,220 | 1.8% |
| 6 | Waterloo | 68,460 | 0.0% |
| 7 | Ames | 65,060 | 2.0% |
| 8 | West Des Moines | 64,113 | 2.5% |
| 9 | Council Bluffs | 62,597 | 0.1% |
| 10 | Dubuque | 58,799 | 0.4% |
| 11 | Ankeny | 56,764 | 4.5% |
| 12 | Urbandale | 44,062 | 2.2% |
| 13 | Cedar Falls | 41,255 | 1.0% |
| 14 | Marion | 37,330 | 1.4% |
| 15 | Bettendorf | 35,505 | 1.3% |
| 16 | Marshalltown | 27,620 | 0.0% |
| 17 | Mason City | 27,366 | -0.5% |
| 18 | Clinton | 26,064 | -0.6% |
| 19 | Burlington | 25,410 | -0.2% |
| 20 | Fort Dodge | 24,649 | -0.4% |
| 21 | Ottumwa | 24,624 | -0.3% |
| 22 | Muscatine | 23,968 | 0.9% |
| 23 | Johnston | 20,871 | 3.9% |
| 24 | Coralville | 20,608 | 1.7% |
| 25 | Waukee | 18,990 | 6.6% |
| 26 | Clive | 17,419 | 2.4% |
| 27 | Altoona | 16,984 | 3.2% |
| 28 | North Liberty | 15,931 | 3.6% |
| 29 | Indianola | 15,467 | 0.9% |
| 30 | Newton | 15,125 | -0.2% |
| 31 | Boone | 12,692 | 0.0% |
| 32 | Oskaloosa | 11,607 | 0.2% |
| 33 | Spencer | 11,212 | -0.0% |
| 34 | Storm Lake | 10,910 | 0.6% |
| 35 | Fort Madison | 10,717 | -0.6% |
| 36 | Grimes | 10,676 | 5.3% |
| 37 | Keokuk | 10,609 | -0.3% |
| 38 | Pella | 10,363 | 0.0% |
| 39 | Norwalk | 10,135 | 2.5% |
| 40 | Waverly | 10,066 | 0.4% |
| 41 | Carroll | 9,968 | -0.3% |
| 42 | Fairfield | 9,892 | 0.9% |
| 43 | Le Mars | 9,761 | -0.1% |
| 44 | Pleasant Hill | 9,314 | 1.2% |
| 45 | Grinnell | 9,141 | -0.2% |
| 46 | Mount Pleasant | 8,433 | -0.5% |
| 47 | Denison | 8,334 | 0.1% |
| 48 | Perry | 8,089 | 1.0% |
| 49 | Decorah | 7,907 | -0.5% |
| 50 | Creston | 7,854 | 0.1% |
| 51 | Webster City | 7,814 | -0.6% |
| 52 | Clear Lake | 7,590 | -0.5% |
| 53 | Sioux Center | 7,461 | 1.1% |
| 54 | Charles City | 7,455 | -0.5% |
| 55 | Washington | 7,408 | 0.4% |
| 56 | Knoxville | 7,248 | -0.2% |
| 57 | Hiawatha | 7,199 | 0.5% |
| 58 | Atlantic | 6,833 | -0.8% |
| 59 | Nevada | 6,831 | 0.1% |
| 60 | Eldridge | 6,232 | 2.0% |
| 61 | Orange City | 6,198 | 0.6% |
| 62 | Oelwein | 6,153 | -0.8% |
| 63 | Independence | 6,028 | 0.2% |
| 64 | Estherville | 6,011 | -1.1% |
| 65 | Maquoketa | 5,989 | -0.5% |
| 66 | Red Oak | 5,472 | -1.0% |
| 67 | Algona | 5,470 | -0.3% |
| 68 | Anamosa | 5,469 | -0.2% |
| 69 | Clarinda | 5,418 | -0.6% |
| 70 | Centerville | 5,372 | -0.6% |
| 71 | Asbury | 5,291 | 4.9% |
| 72 | Glenwood | 5,253 | -0.1% |
| 73 | De Witt | 5,242 | -0.3% |
| 74 | Iowa Falls | 5,195 | -0.2% |
| 75 | Winterset | 5,176 | -0.1% |
| 76 | Vinton | 5,148 | -0.4% |
| 77 | Sheldon | 5,088 | -0.4% |
| 78 | Manchester | 5,073 | -0.4% |
| 79 | Cherokee | 5,030 | -0.9% |
| 80 | Shenandoah | 5,021 | -0.5% |
| 81 | Spirit Lake | 5,018 | 0.7% |
| 82 | Harlan | 5,002 | -0.4% |
| 83 | Bondurant | 4,996 | 5.3% |
| 84 | Osceola | 4,972 | 0.2% |
| 85 | Windsor Heights | 4,889 | 0.1% |
| 86 | Evansdale | 4,812 | 0.3% |
| 87 | Humboldt | 4,596 | -0.4% |
| 88 | Sergeant Bluff | 4,520 | 1.3% |
| 89 | Mount Vernon | 4,486 | -0.1% |
| 90 | Camanche | 4,341 | -0.5% |
| 91 | Polk City | 4,323 | 4.8% |
| 92 | Hampton | 4,290 | -0.8% |
| 93 | Adel | 4,245 | 2.9% |
| 94 | Chariton | 4,214 | -0.5% |
| 95 | Dyersville | 4,204 | 0.7% |
| 96 | Jefferson | 4,204 | -0.7% |
| 97 | Carlisle | 4,168 | 1.5% |
| 98 | Forest City | 4,018 | -0.6% |
| 99 | Le Claire | 3,982 | 1.1% |
| 100 | Cresco | 3,833 | -0.2% |
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