| Rank | City | 2010 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Des Moines | 200,538 | 0.1% |
| 2 | Cedar Rapids | 127,764 | 0.5% |
| 3 | Davenport | 101,335 | 0.3% |
| 4 | Sioux City | 82,903 | -0.3% |
| 5 | Iowa City | 69,086 | 1.1% |
| 6 | Waterloo | 66,761 | -0.3% |
| 7 | Council Bluffs | 60,391 | 0.4% |
| 8 | Dubuque | 57,241 | -0.1% |
| 9 | West Des Moines | 56,954 | 2.1% |
| 10 | Ames | 56,657 | 1.2% |
| 11 | Ankeny | 42,648 | 5.0% |
| 12 | Cedar Falls | 38,714 | 0.7% |
| 13 | Urbandale | 38,677 | 3.1% |
| 14 | Marion | 33,452 | 2.6% |
| 15 | Bettendorf | 33,175 | 0.7% |
| 16 | Mason City | 27,267 | -0.7% |
| 17 | Clinton | 26,459 | -0.5% |
| 18 | Marshalltown | 25,814 | -0.1% |
| 19 | Burlington | 25,527 | -0.5% |
| 20 | Fort Dodge | 25,083 | -0.5% |
| 21 | Ottumwa | 24,329 | -0.3% |
| 22 | Muscatine | 22,653 | -0.0% |
| 23 | Coralville | 18,827 | 2.4% |
| 24 | Johnston | 15,918 | 6.5% |
| 25 | Clive | 15,469 | 2.0% |
| 26 | Newton | 14,841 | -0.6% |
| 27 | Indianola | 14,506 | 1.1% |
| 28 | Altoona | 14,397 | 3.6% |
| 29 | Waukee | 12,826 | 9.7% |
| 30 | Boone | 12,479 | -0.3% |
| 31 | North Liberty | 12,413 | 9.5% |
| 32 | Spencer | 10,975 | -0.4% |
| 33 | Oskaloosa | 10,895 | -0.1% |
| 34 | Fort Madison | 10,870 | -0.8% |
| 35 | Keokuk | 10,406 | -1.0% |
| 36 | Pella | 10,207 | 0.3% |
| 37 | Carroll | 9,877 | -0.2% |
| 38 | Storm Lake | 9,540 | -0.6% |
| 39 | Perry | 9,295 | 2.1% |
| 40 | Fairfield | 9,201 | -0.5% |
| 41 | Waverly | 9,155 | 0.2% |
| 42 | Le Mars | 9,124 | -0.1% |
| 43 | Grinnell | 9,064 | -0.0% |
| 44 | Norwalk | 9,016 | 2.8% |
| 45 | Grimes | 8,794 | 6.2% |
| 46 | Mount Pleasant | 8,673 | -0.1% |
| 47 | Pleasant Hill | 8,424 | 5.4% |
| 48 | Clear Lake | 7,786 | -0.5% |
| 49 | Decorah | 7,765 | -0.6% |
| 50 | Webster City | 7,642 | -0.7% |
| 51 | Creston | 7,556 | -0.0% |
| 52 | Charles City | 7,346 | -0.6% |
| 53 | Knoxville | 7,263 | -0.7% |
| 54 | Washington | 7,197 | 0.2% |
| 55 | Denison | 7,187 | -0.2% |
| 56 | Nevada | 6,736 | 0.1% |
| 57 | Atlantic | 6,728 | -0.8% |
| 58 | Sioux Center | 6,704 | 1.1% |
| 59 | Hiawatha | 6,697 | 0.3% |
| 60 | Estherville | 6,193 | -0.7% |
| 61 | Independence | 6,074 | 0.0% |
| 62 | Oelwein | 5,993 | -1.2% |
| 63 | Orange City | 5,814 | 0.4% |
| 64 | Maquoketa | 5,801 | -0.6% |
| 65 | Anamosa | 5,745 | 0.5% |
| 66 | Glenwood | 5,656 | 0.5% |
| 67 | Red Oak | 5,626 | -1.1% |
| 68 | Centerville | 5,367 | -1.1% |
| 69 | Clarinda | 5,301 | -0.8% |
| 70 | De Witt | 5,243 | 0.4% |
| 71 | Algona | 5,227 | -1.0% |
| 72 | Vinton | 5,096 | -0.0% |
| 73 | Evansdale | 5,068 | 1.3% |
| 74 | Eldridge | 4,948 | 1.8% |
| 75 | Harlan | 4,895 | -0.9% |
| 76 | Iowa Falls | 4,893 | -0.7% |
| 77 | Manchester | 4,872 | -0.9% |
| 78 | Sheldon | 4,838 | -0.4% |
| 79 | Shenandoah | 4,832 | -1.5% |
| 80 | Winterset | 4,830 | 0.2% |
| 81 | Osceola | 4,768 | -0.0% |
| 82 | Cherokee | 4,673 | -1.5% |
| 83 | Windsor Heights | 4,627 | -0.3% |
| 84 | Spirit Lake | 4,594 | 0.8% |
| 85 | Chariton | 4,348 | -0.6% |
| 86 | Adel | 4,321 | 2.5% |
| 87 | Camanche | 4,268 | 0.2% |
| 88 | Humboldt | 4,191 | -0.7% |
| 89 | Mount Vernon | 4,185 | 1.0% |
| 90 | Dyersville | 4,182 | 0.4% |
| 91 | Hampton | 4,152 | -0.1% |
| 92 | Jefferson | 4,117 | -1.3% |
| 93 | Sergeant Bluff | 4,027 | 2.1% |
| 94 | Asbury | 4,022 | 5.5% |
| 95 | Forest City | 3,964 | -1.1% |
| 96 | Waukon | 3,871 | -0.7% |
| 97 | Carlisle | 3,726 | 0.4% |
| 98 | Monticello | 3,701 | 0.2% |
| 99 | Cresco | 3,698 | -0.6% |
| 100 | West Liberty | 3,658 | 1.0% |
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