| Rank | City | 2005 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Memphis | 681,573 | -0.3% |
| 2 | Nashville | 570,068 | 1.1% |
| 3 | Knoxville | 178,124 | 0.3% |
| 4 | Chattanooga | 164,077 | 1.1% |
| 5 | Clarksville | 108,511 | 1.1% |
| 6 | Murfreesboro | 82,713 | 3.9% |
| 7 | Jackson | 61,680 | 0.7% |
| 8 | Johnson City | 57,962 | 0.9% |
| 9 | Franklin | 51,929 | 2.9% |
| 10 | Bartlett | 46,231 | 1.1% |
| 11 | Kingsport | 44,229 | -0.6% |
| 12 | Hendersonville | 43,581 | 1.5% |
| 13 | Germantown | 39,425 | 1.1% |
| 14 | Cleveland | 37,777 | 0.2% |
| 15 | Collierville | 36,489 | 2.6% |
| 16 | Columbia | 33,478 | 0.1% |
| 17 | Smyrna | 32,312 | 3.8% |
| 18 | Brentwood | 30,362 | 3.6% |
| 19 | Cookeville | 28,032 | 1.5% |
| 20 | Oak Ridge | 26,905 | -0.4% |
| 21 | Morristown | 26,684 | 0.2% |
| 22 | Gallatin | 25,755 | 2.3% |
| 23 | Maryville | 25,325 | 2.3% |
| 24 | Bristol | 24,972 | -0.4% |
| 25 | La Vergne | 24,911 | 7.1% |
| 26 | Lebanon | 22,387 | 2.2% |
| 27 | East Ridge | 20,102 | -0.5% |
| 28 | Farragut | 19,304 | 2.1% |
| 29 | Tullahoma | 18,595 | 0.7% |
| 30 | Shelbyville | 17,844 | 2.2% |
| 31 | Dyersburg | 17,258 | -0.3% |
| 32 | Mount Juliet | 17,133 | 4.9% |
| 33 | Springfield | 15,553 | 1.9% |
| 34 | Goodlettsville | 15,465 | 2.9% |
| 35 | Greeneville | 15,161 | -0.4% |
| 36 | Sevierville | 14,503 | 4.2% |
| 37 | Spring Hill | 14,470 | 15.1% |
| 38 | Elizabethton | 13,905 | -0.3% |
| 39 | Athens | 13,561 | 0.5% |
| 40 | Dickson | 13,318 | 0.8% |
| 41 | McMinnville | 13,012 | 0.2% |
| 42 | Soddy-Daisy | 12,062 | 1.1% |
| 43 | Red Bank | 11,902 | -0.9% |
| 44 | Millington | 10,914 | -0.5% |
| 45 | Lawrenceburg | 10,725 | -0.1% |
| 46 | Union City | 10,717 | -0.4% |
| 47 | Brownsville | 10,681 | -0.2% |
| 48 | Lewisburg | 10,674 | 0.2% |
| 49 | Portland | 10,032 | 3.8% |
| 50 | Martin | 10,025 | -1.2% |
| 51 | Crossville | 10,020 | 2.2% |
| 52 | Paris | 9,708 | -0.1% |
| 53 | Clinton | 9,300 | -0.4% |
| 54 | Humboldt | 9,265 | -0.5% |
| 55 | Manchester | 9,235 | 1.9% |
| 56 | Covington | 8,887 | 1.1% |
| 57 | Alcoa | 8,447 | 1.3% |
| 58 | White House | 8,405 | 2.9% |
| 59 | La Follette | 8,120 | 0.4% |
| 60 | Jefferson City | 7,933 | 0.1% |
| 61 | Pulaski | 7,882 | -0.4% |
| 62 | Milan | 7,787 | -0.3% |
| 63 | Ripley | 7,694 | -0.4% |
| 64 | Lakeland | 7,626 | 1.3% |
| 65 | Winchester | 7,599 | 0.8% |
| 66 | Lexington | 7,484 | 0.2% |
| 67 | Lenoir City | 7,440 | 1.9% |
| 68 | Hartsville/Trousdale County | 7,301 | -0.0% |
| 69 | Collegedale | 7,285 | 2.1% |
| 70 | Signal Mountain | 7,282 | -0.5% |
| 71 | Newport | 7,269 | -0.2% |
| 72 | Savannah | 7,099 | 0.3% |
| 73 | Fairview | 6,935 | 3.2% |
| 74 | Fayetteville | 6,934 | -0.3% |
| 75 | Harriman | 6,712 | -0.2% |
| 76 | Dayton | 6,532 | 0.0% |
| 77 | Church Hill | 6,174 | 0.9% |
| 78 | Millersville | 6,161 | 3.0% |
| 79 | Sweetwater | 5,971 | 1.1% |
| 80 | Henderson | 5,950 | 0.3% |
| 81 | Lynchburg, Moore County | 5,923 | 0.7% |
| 82 | Greenbrier | 5,868 | 4.0% |
| 83 | Erwin | 5,813 | -0.1% |
| 84 | Pigeon Forge | 5,777 | 1.9% |
| 85 | Bolivar | 5,608 | -1.0% |
| 86 | Munford | 5,514 | 2.7% |
| 87 | McKenzie | 5,432 | 0.6% |
| 88 | Kingston | 5,404 | 0.4% |
| 89 | Rockwood | 5,386 | -0.2% |
| 90 | Forest Hills | 5,253 | 2.7% |
| 91 | Atoka | 5,188 | 8.3% |
| 92 | Mount Carmel | 5,157 | 1.7% |
| 93 | Dunlap | 4,880 | 1.9% |
| 94 | Oak Hill | 4,879 | 2.0% |
| 95 | Loudon | 4,768 | 0.8% |
| 96 | Sparta | 4,695 | 0.5% |
| 97 | Jonesborough | 4,635 | 1.7% |
| 98 | Trenton | 4,577 | -0.7% |
| 99 | Whiteville | 4,523 | -0.4% |
| 100 | Selmer | 4,509 | -0.1% |
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