| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Memphis | 690,186 | 1.1% |
| 2 | Nashville | 545,902 | 1.1% |
| 3 | Knoxville | 176,273 | 0.2% |
| 4 | Chattanooga | 156,770 | 0.3% |
| 5 | Clarksville | 103,944 | 3.1% |
| 6 | Murfreesboro | 70,858 | 4.5% |
| 7 | Jackson | 59,922 | 1.5% |
| 8 | Johnson City | 55,999 | 0.8% |
| 9 | Franklin | 46,376 | 8.5% |
| 10 | Kingsport | 45,383 | 0.7% |
| 11 | Bartlett | 44,210 | 4.7% |
| 12 | Hendersonville | 41,046 | 2.3% |
| 13 | Germantown | 37,796 | 1.4% |
| 14 | Cleveland | 37,479 | 1.4% |
| 15 | Columbia | 33,286 | 1.4% |
| 16 | Collierville | 32,990 | 8.4% |
| 17 | Smyrna | 27,796 | 5.4% |
| 18 | Oak Ridge | 27,306 | -0.0% |
| 19 | Morristown | 26,489 | 1.5% |
| 20 | Cookeville | 26,398 | 1.8% |
| 21 | Brentwood | 26,340 | 4.9% |
| 22 | Bristol | 25,421 | 0.2% |
| 23 | Gallatin | 23,559 | 2.2% |
| 24 | Maryville | 23,165 | 1.3% |
| 25 | East Ridge | 20,533 | -0.3% |
| 26 | Lebanon | 20,502 | 1.7% |
| 27 | La Vergne | 18,946 | 9.3% |
| 28 | Tullahoma | 18,072 | 0.5% |
| 29 | Farragut | 17,789 | 3.2% |
| 30 | Dyersburg | 17,486 | 0.4% |
| 31 | Shelbyville | 16,342 | 1.1% |
| 32 | Greeneville | 15,390 | 0.0% |
| 33 | Springfield | 14,441 | 1.8% |
| 34 | Mount Juliet | 14,172 | 5.5% |
| 35 | Elizabethton | 14,054 | 0.4% |
| 36 | Goodlettsville | 13,772 | 2.3% |
| 37 | Athens | 13,299 | 0.8% |
| 38 | Dickson | 12,913 | 1.8% |
| 39 | McMinnville | 12,893 | 0.5% |
| 40 | Red Bank | 12,339 | 0.0% |
| 41 | Sevierville | 12,307 | 4.8% |
| 42 | Soddy-Daisy | 11,542 | 1.5% |
| 43 | Millington | 11,125 | -5.0% |
| 44 | Union City | 10,892 | 0.4% |
| 45 | Lawrenceburg | 10,766 | -0.3% |
| 46 | Brownsville | 10,766 | 0.3% |
| 47 | Lewisburg | 10,598 | 0.6% |
| 48 | Martin | 10,540 | 0.9% |
| 49 | Paris | 9,752 | -1.2% |
| 50 | Humboldt | 9,464 | -0.2% |
| 51 | Clinton | 9,460 | -0.1% |
| 52 | Crossville | 9,175 | 2.4% |
| 53 | Portland | 8,642 | 4.2% |
| 54 | Manchester | 8,565 | -0.2% |
| 55 | Covington | 8,521 | 0.6% |
| 56 | Spring Hill | 8,251 | 18.3% |
| 57 | Alcoa | 8,033 | -0.7% |
| 58 | Pulaski | 8,000 | -0.1% |
| 59 | La Follette | 7,979 | 0.6% |
| 60 | Jefferson City | 7,895 | 2.5% |
| 61 | Milan | 7,889 | 0.6% |
| 62 | Ripley | 7,817 | 0.6% |
| 63 | White House | 7,493 | 8.7% |
| 64 | Signal Mountain | 7,440 | 0.5% |
| 65 | Lexington | 7,436 | 0.2% |
| 66 | Winchester | 7,359 | 0.4% |
| 67 | Newport | 7,318 | -0.1% |
| 68 | Hartsville/Trousdale County | 7,308 | – |
| 69 | Lakeland | 7,228 | 10.6% |
| 70 | Savannah | 7,026 | 0.8% |
| 71 | Fayetteville | 7,024 | -0.3% |
| 72 | Lenoir City | 6,901 | 1.0% |
| 73 | Harriman | 6,771 | -0.5% |
| 74 | Collegedale | 6,707 | 2.3% |
| 75 | Dayton | 6,522 | 1.2% |
| 76 | Fairview | 6,120 | 3.5% |
| 77 | Church Hill | 5,954 | 1.2% |
| 78 | Henderson | 5,877 | 1.4% |
| 79 | Erwin | 5,840 | 0.3% |
| 80 | Bolivar | 5,827 | -0.6% |
| 81 | Lynchburg, Moore County | 5,765 | 2.1% |
| 82 | Sweetwater | 5,710 | 1.1% |
| 83 | Millersville | 5,472 | 4.7% |
| 84 | Rockwood | 5,429 | -0.9% |
| 85 | Pigeon Forge | 5,365 | 3.4% |
| 86 | McKenzie | 5,310 | -0.2% |
| 87 | Kingston | 5,309 | 0.8% |
| 88 | Greenbrier | 5,015 | 5.1% |
| 89 | Munford | 4,964 | 5.4% |
| 90 | Mount Carmel | 4,823 | 1.2% |
| 91 | Forest Hills | 4,731 | 1.1% |
| 92 | Trenton | 4,704 | -0.3% |
| 93 | Loudon | 4,616 | 0.7% |
| 94 | Sparta | 4,611 | -0.3% |
| 95 | Whiteville | 4,590 | 14.1% |
| 96 | Selmer | 4,524 | -1.0% |
| 97 | Dunlap | 4,522 | 1.9% |
| 98 | Oak Hill | 4,502 | 0.4% |
| 99 | Mount Pleasant | 4,490 | 0.0% |
| 100 | Huntingdon | 4,373 | -1.0% |
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