| Rank | City | 2020 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Summerset | 2,995 | 5.1% |
| 2 | Harrisburg | 6,843 | 5.3% |
| 3 | Piedmont | 972 | 15.9% |
| 4 | Box Elder | 11,923 | 4.3% |
| 5 | Tea | 5,677 | 4.1% |
| 6 | Akaska | 73 | 5.7% |
| 7 | Aurora | 1,049 | 7.0% |
| 8 | Brandon | 10,986 | 2.3% |
| 9 | Cottonwood | 11 | 2.0% |
| 10 | Hartford | 3,347 | 2.8% |
| 11 | Crooks | 1,352 | 0.6% |
| 12 | Worthing | 930 | 0.6% |
| 13 | Camp Crook | 85 | 3.0% |
| 14 | Sioux Falls | 193,233 | 2.3% |
| 15 | Baltic | 1,246 | 1.4% |
| 16 | Naples | 37 | -1.0% |
| 17 | Volga | 2,099 | 1.7% |
| 18 | Quinn | 63 | 1.6% |
| 19 | Spearfish | 12,201 | 1.5% |
| 20 | Eagle Butte | 1,260 | -0.4% |
| 21 | Lake Norden | 596 | 2.5% |
| 22 | Pickstown | 232 | 1.4% |
| 23 | Vilas | 26 | 2.7% |
| 24 | Brentford | 88 | 1.3% |
| 25 | North Sioux City | 3,043 | 1.9% |
| 26 | Dell Rapids | 3,980 | 0.9% |
| 27 | Springfield | 2,053 | 0.3% |
| 28 | Plankinton | 774 | 0.9% |
| 29 | Mission | 1,145 | -0.3% |
| 30 | Brookings | 23,430 | 0.6% |
| 31 | Elk Point | 2,135 | 0.8% |
| 32 | Rapid City | 74,350 | 0.9% |
| 33 | Huron | 14,245 | 1.2% |
| 34 | Hermosa | 375 | -0.6% |
| 35 | Lennox | 2,425 | 1.4% |
| 36 | Fruitdale | 73 | 1.3% |
| 37 | Dupree | 498 | -0.5% |
| 38 | Bryant | 461 | 0.1% |
| 39 | Belle Fourche | 5,601 | 0.0% |
| 40 | Estelline | 787 | 0.2% |
| 41 | Parker | 1,209 | 1.7% |
| 42 | Timber Lake | 511 | 1.4% |
| 43 | Veblen | 323 | -4.8% |
| 44 | Warner | 481 | 0.5% |
| 45 | Yankton | 15,399 | 0.6% |
| 46 | Vermillion | 11,678 | 1.0% |
| 47 | Northville | 140 | -0.2% |
| 48 | Hill City | 888 | -0.7% |
| 49 | Aberdeen | 28,442 | 0.9% |
| 50 | Watertown | 22,613 | 0.5% |
| 51 | Columbia | 156 | 1.4% |
| 52 | Colman | 633 | 0.6% |
| 53 | Elkton | 751 | 0.2% |
| 54 | Colton | 734 | 0.7% |
| 55 | Florence | 329 | -1.3% |
| 56 | Valley Springs | 875 | 1.4% |
| 57 | Wolsey | 458 | 2.0% |
| 58 | Sturgis | 7,093 | 0.7% |
| 59 | Wallace | 94 | 1.0% |
| 60 | Humboldt | 577 | -0.2% |
| 61 | Beresford | 2,178 | 0.8% |
| 62 | Wetonka | 13 | 5.0% |
| 63 | Ethan | 356 | 0.7% |
| 64 | Iroquois | 300 | 1.2% |
| 65 | New Witten | 55 | -3.6% |
| 66 | Mitchell | 15,683 | 0.3% |
| 67 | Hayti | 389 | 0.2% |
| 68 | Faulkton | 828 | 1.2% |
| 69 | Chamberlain | 2,485 | 0.4% |
| 70 | Alexandria | 597 | -0.3% |
| 71 | Whitewood | 888 | -0.4% |
| 72 | Pierpont | 127 | -0.6% |
| 73 | Gary | 242 | 0.6% |
| 74 | Garretson | 1,222 | 0.5% |
| 75 | Virgil | 26 | 5.0% |
| 76 | Fort Pierre | 2,119 | 0.2% |
| 77 | Mission Hill | 188 | 0.6% |
| 78 | Brandt | 116 | 0.8% |
| 79 | Toronto | 197 | -0.7% |
| 80 | Castlewood | 684 | 0.9% |
| 81 | Groton | 1,385 | -0.5% |
| 82 | Stockholm | 107 | -0.1% |
| 83 | Pierre | 14,023 | 0.3% |
| 84 | Freeman | 1,327 | 0.2% |
| 85 | Woonsocket | 726 | 1.0% |
| 86 | Avon | 565 | -0.4% |
| 87 | Centerville | 915 | 0.4% |
| 88 | New Effington | 234 | -0.9% |
| 89 | Summit | 286 | -0.1% |
| 90 | Emery | 442 | -0.1% |
| 91 | Verdon | 6 | 1.8% |
| 92 | Flandreau | 2,370 | 0.1% |
| 93 | Custer | 1,966 | -0.5% |
| 94 | Langford | 290 | -0.8% |
| 95 | White | 529 | 0.9% |
| 96 | Hitchcock | 107 | 1.6% |
| 97 | Irene | 427 | 0.2% |
| 98 | Kennebec | 283 | 1.7% |
| 99 | Lead | 2,977 | -0.5% |
| 100 | Nisland | 207 | -1.1% |
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