| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Charlotte | 569,858 | 2.9% |
| 2 | Raleigh | 290,184 | 2.8% |
| 3 | Greensboro | 228,518 | 1.8% |
| 4 | Winston-Salem | 202,198 | 1.9% |
| 5 | Fayetteville | 199,429 | 5.9% |
| 6 | Durham | 188,870 | 2.4% |
| 7 | Cary | 97,411 | 7.9% |
| 8 | Wilmington | 89,765 | 4.8% |
| 9 | High Point | 86,609 | 2.2% |
| 10 | Asheville | 72,883 | 1.0% |
| 11 | Gastonia | 67,622 | 1.2% |
| 12 | Jacksonville | 65,776 | -1.7% |
| 13 | Greenville | 62,791 | 2.4% |
| 14 | Rocky Mount | 58,819 | 0.8% |
| 15 | Concord | 56,620 | 4.2% |
| 16 | Chapel Hill | 48,032 | 2.3% |
| 17 | Burlington | 45,934 | 1.2% |
| 18 | Wilson | 45,060 | 1.4% |
| 19 | Goldsboro | 40,165 | -1.1% |
| 20 | Hickory | 37,690 | 2.5% |
| 21 | Kannapolis | 37,391 | 1.4% |
| 22 | Salisbury | 30,582 | 2.3% |
| 23 | Monroe | 27,388 | 3.7% |
| 24 | Huntersville | 26,145 | 14.8% |
| 25 | Thomasville | 24,512 | 4.0% |
| 26 | Kinston | 23,819 | -0.8% |
| 27 | Statesville | 23,532 | 1.0% |
| 28 | Sanford | 23,455 | 2.2% |
| 29 | New Bern | 23,270 | 1.1% |
| 30 | Matthews | 23,081 | 4.8% |
| 31 | Havelock | 22,464 | 0.9% |
| 32 | Asheboro | 22,204 | 2.0% |
| 33 | Apex | 21,233 | 14.5% |
| 34 | Lumberton | 21,121 | 0.7% |
| 35 | Shelby | 20,851 | 0.5% |
| 36 | Lexington | 19,922 | 0.3% |
| 37 | Mooresville | 19,494 | 5.7% |
| 38 | Kernersville | 18,849 | 4.6% |
| 39 | Garner | 18,832 | 2.4% |
| 40 | Lenoir | 18,133 | 0.8% |
| 41 | Carrboro | 17,703 | 4.2% |
| 42 | Morganton | 17,418 | 0.9% |
| 43 | Mint Hill | 17,277 | 2.4% |
| 44 | Elizabeth City | 17,242 | 0.7% |
| 45 | Roanoke Rapids | 16,991 | 0.4% |
| 46 | Henderson | 16,349 | -0.1% |
| 47 | Albemarle | 16,022 | 0.5% |
| 48 | Laurinburg | 15,967 | -0.1% |
| 49 | Eden | 15,852 | -0.0% |
| 50 | Clemmons | 15,825 | 5.6% |
| 51 | Reidsville | 14,652 | 0.1% |
| 52 | Cornelius | 14,171 | 10.8% |
| 53 | Indian Trail | 13,622 | 12.4% |
| 54 | Boone | 13,534 | 0.4% |
| 55 | Graham | 13,233 | 2.5% |
| 56 | Wake Forest | 13,076 | 8.1% |
| 57 | Newton | 12,713 | 1.3% |
| 58 | Spring Lake | 12,496 | 4.1% |
| 59 | Hope Mills | 12,189 | 4.1% |
| 60 | Lewisville | 11,722 | 5.9% |
| 61 | Hendersonville | 11,440 | 2.4% |
| 62 | Tarboro | 11,223 | 0.1% |
| 63 | Southern Pines | 11,111 | 1.4% |
| 64 | Smithfield | 11,037 | 0.3% |
| 65 | Mount Airy | 10,880 | 3.5% |
| 66 | Kings Mountain | 10,287 | 1.6% |
| 67 | Holly Springs | 10,123 | 22.1% |
| 68 | Lincolnton | 10,103 | 0.8% |
| 69 | Pinehurst | 9,809 | 5.5% |
| 70 | Washington | 9,726 | -0.2% |
| 71 | Mount Holly | 9,679 | 2.0% |
| 72 | Waynesville | 9,581 | 0.6% |
| 73 | Rockingham | 9,558 | -0.4% |
| 74 | Dunn | 9,293 | 0.1% |
| 75 | Archdale | 9,098 | 2.2% |
| 76 | Belmont | 8,897 | -0.2% |
| 77 | Roxboro | 8,709 | 1.4% |
| 78 | Clinton | 8,621 | -0.2% |
| 79 | Clayton | 8,594 | 6.2% |
| 80 | Oxford | 8,417 | 0.0% |
| 81 | Fuquay-Varina | 8,198 | 6.2% |
| 82 | Morehead City | 7,885 | 0.4% |
| 83 | Mebane | 7,641 | 4.6% |
| 84 | Black Mountain | 7,544 | 0.4% |
| 85 | Forest City | 7,531 | -0.3% |
| 86 | Davidson | 7,260 | 5.7% |
| 87 | Stallings | 7,206 | 12.7% |
| 88 | Siler City | 7,095 | 3.4% |
| 89 | Summerfield | 6,874 | 15.3% |
| 90 | Marion | 6,867 | 3.5% |
| 91 | Elon | 6,817 | – |
| 92 | Brevard | 6,811 | 0.9% |
| 93 | Weddington | 6,774 | 5.2% |
| 94 | Conover | 6,754 | 2.0% |
| 95 | Trinity | 6,724 | 0.4% |
| 96 | Oak Island | 6,668 | – |
| 97 | Butner | 6,454 | – |
| 98 | Knightdale | 6,312 | 11.4% |
| 99 | Hamlet | 6,206 | -1.4% |
| 100 | Selma | 6,098 | 1.5% |
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