| Rank | City | 2020 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Portland | 66,215 | 0.0% |
| 2 | Lewiston | 36,225 | -0.1% |
| 3 | Bangor | 32,262 | -0.3% |
| 4 | South Portland | 25,532 | 0.2% |
| 5 | Auburn | 23,414 | 0.2% |
| 6 | Biddeford | 21,504 | 0.1% |
| 7 | Sanford | 21,223 | – |
| 8 | Saco | 19,964 | 0.9% |
| 9 | Westbrook | 19,074 | 1.0% |
| 10 | Augusta | 18,697 | -0.3% |
| 11 | Waterville | 16,558 | 0.6% |
| 12 | Brewer | 9,035 | -0.5% |
| 13 | Presque Isle | 9,007 | -0.8% |
| 14 | Bath | 8,338 | -0.2% |
| 15 | Ellsworth | 8,180 | 0.6% |
| 16 | Caribou | 7,593 | -0.8% |
| 17 | Old Town | 7,431 | -0.6% |
| 18 | Rockland | 7,165 | -0.2% |
| 19 | Belfast | 6,679 | 0.0% |
| 20 | Gardiner | 5,653 | -0.3% |
| 21 | Calais | 3,005 | -0.4% |
| 22 | Hallowell | 2,381 | – |
| 23 | Eastport | 1,265 | -0.6% |
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