| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Boston | 590,433 | 0.3% |
| 2 | Worcester | 172,500 | 0.2% |
| 3 | Springfield | 151,895 | -0.3% |
| 4 | Lowell | 105,181 | 0.2% |
| 5 | Cambridge | 101,683 | 0.6% |
| 6 | Brockton | 94,455 | 0.2% |
| 7 | New Bedford | 93,896 | -0.5% |
| 8 | Fall River | 92,116 | -0.1% |
| 9 | Lynn | 89,210 | 0.9% |
| 10 | Quincy | 88,351 | 0.4% |
| 11 | Newton | 83,788 | 0.1% |
| 12 | Somerville | 77,510 | 0.2% |
| 13 | Lawrence | 72,176 | 0.3% |
| 14 | Waltham | 59,214 | 0.2% |
| 15 | Haverhill | 59,113 | 1.5% |
| 16 | Malden | 56,296 | 0.4% |
| 17 | Taunton | 56,119 | 1.2% |
| 18 | Medford | 55,662 | -0.3% |
| 19 | Chicopee | 54,735 | -0.3% |
| 20 | Peabody | 48,376 | 0.3% |
| 21 | Barnstable Town | 48,054 | 1.6% |
| 22 | Revere | 47,383 | 1.0% |
| 23 | Pittsfield | 45,704 | -0.7% |
| 24 | Methuen | 43,952 | 0.9% |
| 25 | Attleboro | 41,954 | 0.9% |
| 26 | Leominster | 41,469 | 0.9% |
| 27 | Salem | 40,450 | 0.6% |
| 28 | Westfield | 40,084 | 0.4% |
| 29 | Holyoke | 39,883 | -0.9% |
| 30 | Beverly | 39,842 | 0.4% |
| 31 | Fitchburg | 39,010 | -0.7% |
| 32 | Everett | 38,291 | 0.7% |
| 33 | Woburn | 37,420 | 0.4% |
| 34 | Marlborough | 36,612 | 1.4% |
| 35 | Chelsea | 35,105 | 2.0% |
| 36 | Watertown | 33,003 | -0.1% |
| 37 | Gloucester | 30,357 | 0.6% |
| 38 | Franklin | 29,633 | 3.0% |
| 39 | Northampton | 28,974 | -0.1% |
| 40 | Agawam | 28,184 | 0.3% |
| 41 | West Springfield | 27,936 | – |
| 42 | Melrose | 27,183 | -0.3% |
| 43 | Gardner | 20,839 | 0.3% |
| 44 | Newburyport | 17,183 | 0.5% |
| 45 | Easthampton | 16,003 | – |
| 46 | North Adams | 14,651 | -1.5% |
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