| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Jacksonville | 736,583 | 1.5% |
| 2 | Miami | 363,177 | 0.1% |
| 3 | Tampa | 304,157 | 0.8% |
| 4 | St. Petersburg | 248,751 | 0.3% |
| 5 | Hialeah | 226,142 | 1.9% |
| 6 | Orlando | 194,723 | 1.8% |
| 7 | Fort Lauderdale | 171,099 | 1.4% |
| 8 | Tallahassee | 152,967 | 2.0% |
| 9 | Hollywood | 139,703 | 1.3% |
| 10 | Pembroke Pines | 138,241 | 7.8% |
| 11 | Coral Springs | 119,733 | 4.2% |
| 12 | Gainesville | 112,997 | 2.0% |
| 13 | Clearwater | 109,527 | 1.2% |
| 14 | Cape Coral | 103,337 | 3.2% |
| 15 | Miami Gardens | 100,758 | – |
| 16 | Pompano Beach | 100,326 | 3.3% |
| 17 | Port St. Lucie | 89,175 | 4.8% |
| 18 | Miami Beach | 88,055 | -0.5% |
| 19 | Sunrise | 86,071 | 2.7% |
| 20 | Lakeland | 85,067 | 1.8% |
| 21 | Boca Raton | 83,146 | 2.8% |
| 22 | Plantation | 83,085 | 2.3% |
| 23 | West Palm Beach | 82,879 | 2.2% |
| 24 | Davie | 82,202 | 4.8% |
| 25 | Palm Bay | 79,625 | 2.4% |
| 26 | Miramar | 74,554 | 6.1% |
| 27 | Largo | 74,539 | 1.1% |
| 28 | Deerfield Beach | 74,424 | 4.7% |
| 29 | Melbourne | 73,153 | 1.9% |
| 30 | Deltona | 70,080 | 3.6% |
| 31 | Lauderhill | 66,940 | 3.2% |
| 32 | Daytona Beach | 63,808 | 0.2% |
| 33 | Boynton Beach | 60,580 | 2.6% |
| 34 | Delray Beach | 60,332 | 2.4% |
| 35 | North Miami | 59,762 | 1.8% |
| 36 | Pensacola | 56,092 | -0.5% |
| 37 | Tamarac | 55,929 | 2.3% |
| 38 | Margate | 53,976 | 2.3% |
| 39 | Sarasota | 52,541 | 0.3% |
| 40 | Fort Myers | 51,971 | 1.5% |
| 41 | Weston | 50,813 | 17.9% |
| 42 | Bradenton | 50,044 | 1.4% |
| 43 | Kissimmee | 49,143 | 4.8% |
| 44 | Ocala | 47,538 | 1.1% |
| 45 | Pinellas Park | 47,169 | 0.7% |
| 46 | Port Orange | 46,575 | 2.3% |
| 47 | Coconut Creek | 44,227 | 5.0% |
| 48 | Coral Gables | 42,732 | 0.3% |
| 49 | Oakland Park | 42,128 | 5.1% |
| 50 | North Miami Beach | 41,733 | 1.8% |
| 51 | Altamonte Springs | 41,295 | 1.6% |
| 52 | Titusville | 40,990 | 0.3% |
| 53 | Fort Pierce | 40,282 | 0.7% |
| 54 | Jupiter | 39,874 | 3.6% |
| 55 | Sanford | 39,193 | 1.9% |
| 56 | Wellington | 38,833 | 6.0% |
| 57 | North Lauderdale | 38,581 | 3.7% |
| 58 | Panama City | 36,818 | 0.5% |
| 59 | Ormond Beach | 36,539 | 1.5% |
| 60 | Palm Beach Gardens | 36,300 | 4.3% |
| 61 | Dunedin | 36,266 | 0.3% |
| 62 | Lake Worth | 35,632 | 2.1% |
| 63 | Hallandale Beach | 34,368 | 1.3% |
| 64 | Palm Coast | 33,188 | – |
| 65 | Bonita Springs | 33,042 | – |
| 66 | Homestead | 31,914 | 1.2% |
| 67 | Lauderdale Lakes | 31,238 | 1.1% |
| 68 | Winter Springs | 31,129 | 3.4% |
| 69 | Cutler Bay | 30,307 | – |
| 70 | Plant City | 30,214 | 2.8% |
| 71 | Riviera Beach | 29,893 | 0.9% |
| 72 | Greenacres | 28,537 | 3.9% |
| 73 | Cooper City | 28,423 | 3.5% |
| 74 | Winter Park | 27,879 | 1.1% |
| 75 | Apopka | 27,811 | 6.3% |
| 76 | Winter Haven | 27,417 | 0.4% |
| 77 | Dania Beach | 27,367 | – |
| 78 | Oviedo | 26,441 | 8.8% |
| 79 | Key West | 25,431 | 0.2% |
| 80 | Aventura | 25,266 | 5.1% |
| 81 | Ocoee | 24,806 | 6.6% |
| 82 | Casselberry | 24,477 | 1.4% |
| 83 | Palmetto Bay | 23,825 | – |
| 84 | North Port | 23,249 | 6.9% |
| 85 | Miami Lakes | 22,662 | – |
| 86 | Royal Palm Beach | 21,973 | 3.5% |
| 87 | St. Cloud | 21,967 | 5.5% |
| 88 | Temple Terrace | 21,862 | 1.8% |
| 89 | Naples | 21,501 | 0.8% |
| 90 | Tarpon Springs | 21,383 | 1.2% |
| 91 | De Land | 21,251 | 2.2% |
| 92 | Doral | 21,130 | – |
| 93 | Jacksonville Beach | 20,997 | 1.5% |
| 94 | New Smyrna Beach | 20,576 | 1.8% |
| 95 | Rockledge | 20,381 | 2.4% |
| 96 | Fort Walton Beach | 20,014 | -0.6% |
| 97 | Hialeah Gardens | 19,314 | 9.6% |
| 98 | Pinecrest | 19,052 | 0.6% |
| 99 | Edgewater | 18,891 | 2.0% |
| 100 | Seminole | 18,652 | 7.3% |
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