| Rank | City | 2023 Population (Est.) | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Huntsville | 222,030 | 1.6% | 
| 2 | Montgomery | 196,944 | -0.8% | 
| 3 | Birmingham | 196,887 | -0.8% | 
| 4 | Mobile | 183,290 | -0.8% | 
| 5 | Tuscaloosa | 110,575 | 5.2% | 
| 6 | Hoover | 92,406 | -0.2% | 
| 7 | Auburn | 80,377 | 2.6% | 
| 8 | Dothan | 71,211 | 0.0% | 
| 9 | Madison | 59,791 | 2.1% | 
| 10 | Decatur | 57,929 | 0.1% | 
| 11 | Florence | 41,687 | 2.3% | 
| 12 | Prattville | 38,754 | 1.1% | 
| 13 | Phenix City | 38,289 | -0.6% | 
| 14 | Vestavia Hills | 38,286 | -1.0% | 
| 15 | Alabaster | 33,869 | 0.7% | 
| 16 | Gadsden | 33,276 | -1.1% | 
| 17 | Opelika | 32,771 | 2.7% | 
| 18 | Northport | 31,023 | -0.2% | 
| 19 | Enterprise | 29,784 | 1.6% | 
| 20 | Daphne | 29,667 | 3.4% | 
| 21 | Athens | 28,642 | 5.6% | 
| 22 | Homewood | 27,852 | 2.9% | 
| 23 | Trussville | 26,499 | 0.5% | 
| 24 | Bessemer | 25,288 | -1.3% | 
| 25 | Pelham | 24,746 | 0.6% | 
| 26 | Fairhope | 23,866 | 2.8% | 
| 27 | Foley | 23,693 | 7.2% | 
| 28 | Albertville | 22,726 | 0.8% | 
| 29 | Oxford | 22,004 | -0.7% | 
| 30 | Mountain Brook | 21,941 | -1.0% | 
| 31 | Helena | 21,896 | 1.9% | 
| 32 | Anniston | 21,181 | -0.3% | 
| 33 | Cullman | 19,265 | 2.8% | 
| 34 | Prichard | 18,869 | -1.0% | 
| 35 | Calera | 17,853 | 3.5% | 
| 36 | Troy | 17,777 | 0.6% | 
| 37 | Millbrook | 17,104 | 0.7% | 
| 38 | Muscle Shoals | 17,026 | 2.1% | 
| 39 | Selma | 17,007 | -2.7% | 
| 40 | Saraland | 16,410 | 0.7% | 
| 41 | Hueytown | 16,356 | -1.2% | 
| 42 | Gulf Shores | 16,281 | 3.4% | 
| 43 | Chelsea | 16,215 | 3.4% | 
| 44 | Gardendale | 16,213 | 0.5% | 
| 45 | Center Point | 15,880 | -1.5% | 
| 46 | Scottsboro | 15,690 | 0.4% | 
| 47 | Hartselle | 15,653 | 0.6% | 
| 48 | Fort Payne | 14,894 | 0.2% | 
| 49 | Talladega | 14,810 | -3.2% | 
| 50 | Alexander City | 14,620 | -0.5% | 
| 51 | Jacksonville | 14,431 | 5.0% | 
| 52 | Jasper | 14,367 | -0.1% | 
| 53 | Ozark | 14,286 | -0.1% | 
| 54 | Pell City | 13,628 | 2.8% | 
| 55 | Moody | 13,581 | 1.2% | 
| 56 | Irondale | 13,356 | -0.6% | 
| 57 | Eufaula | 12,535 | -1.1% | 
| 58 | Leeds | 12,339 | 0.0% | 
| 59 | Sylacauga | 12,257 | -1.1% | 
| 60 | Russellville | 10,771 | -0.2% | 
| 61 | Pike Road | 10,687 | 5.7% | 
| 62 | Spanish Fort | 10,655 | 2.7% | 
| 63 | Valley | 10,343 | -1.0% | 
| 64 | Clay | 10,288 | -0.1% | 
| 65 | Boaz | 10,283 | 0.9% | 
| 66 | Rainbow City | 10,259 | 0.5% | 
| 67 | Fairfield | 9,689 | -1.4% | 
| 68 | Fultondale | 9,678 | -1.0% | 
| 69 | Southside | 9,538 | 0.7% | 
| 70 | Pleasant Grove | 9,441 | -0.5% | 
| 71 | Sheffield | 9,307 | -0.4% | 
| 72 | Tuscumbia | 9,106 | 0.3% | 
| 73 | Clanton | 8,951 | 1.3% | 
| 74 | Guntersville | 8,871 | 1.7% | 
| 75 | Tuskegee | 8,860 | -1.6% | 
| 76 | Andalusia | 8,791 | 0.3% | 
| 77 | Arab | 8,626 | 1.1% | 
| 78 | Orange Beach | 8,450 | 1.7% | 
| 79 | Atmore | 8,329 | -1.3% | 
| 80 | Bay Minette | 8,279 | 3.1% | 
| 81 | Pinson | 7,791 | 3.6% | 
| 82 | Montevallo | 7,632 | 4.4% | 
| 83 | Wetumpka | 7,359 | 2.1% | 
| 84 | Lincoln | 7,260 | 2.5% | 
| 85 | Robertsdale | 7,194 | 3.5% | 
| 86 | Greenville | 7,165 | -1.3% | 
| 87 | Oneonta | 6,969 | 1.1% | 
| 88 | Hamilton | 6,957 | -0.4% | 
| 89 | Demopolis | 6,899 | -1.7% | 
| 90 | Satsuma | 6,802 | 0.3% | 
| 91 | Lanett | 6,780 | -0.4% | 
| 92 | Opp | 6,719 | 0.4% | 
| 93 | Chickasaw | 6,315 | -1.1% | 
| 94 | Tarrant | 5,905 | -1.6% | 
| 95 | Attalla | 5,828 | -0.1% | 
| 96 | Margaret | 5,812 | 5.9% | 
| 97 | Monroeville | 5,804 | -0.7% | 
| 98 | Rainsville | 5,682 | 1.4% | 
| 99 | Semmes | 5,603 | 5.9% | 
| 100 | Smiths Station | 5,483 | 0.8% | 
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