| Rank | City | 1900 Population | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Mobile | 38,469 | 2.2% | 
| 2 | Birmingham | 38,415 | – | 
In 1900, the largest city in Alabama was Mobile, with a population of 38,469. The second largest city is Birmingham, with a population of 38,415.
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