| Rank | City | 2010 Population (Est.) | Growth Rate (Annual) | 
|---|---|---|---|
| 1 | Birmingham | 230,131 | -0.6% | 
| 2 | Montgomery | 202,124 | 0.0% | 
| 3 | Mobile | 193,205 | -0.3% | 
| 4 | Huntsville | 179,652 | 1.3% | 
| 5 | Tuscaloosa | 93,138 | 1.9% | 
| 6 | Hoover | 72,989 | 1.6% | 
| 7 | Dothan | 67,525 | 1.6% | 
| 8 | Auburn | 57,828 | 2.5% | 
| 9 | Decatur | 56,411 | 0.5% | 
| 10 | Madison | 39,875 | 3.3% | 
| 11 | Florence | 38,119 | 0.5% | 
| 12 | Gadsden | 36,719 | -0.6% | 
| 13 | Prattville | 32,696 | 2.5% | 
| 14 | Vestavia Hills | 31,121 | 0.1% | 
| 15 | Phenix City | 31,100 | 1.0% | 
| 16 | Alabaster | 29,861 | 2.4% | 
| 17 | Bessemer | 28,772 | -0.6% | 
| 18 | Prichard | 27,576 | -0.4% | 
| 19 | Opelika | 27,454 | 1.2% | 
| 20 | Enterprise | 25,885 | 2.3% | 
| 21 | Athens | 24,194 | 2.6% | 
| 22 | Homewood | 23,993 | -0.5% | 
| 23 | Anniston | 23,561 | -0.3% | 
| 24 | Northport | 23,422 | 1.8% | 
| 25 | Pelham | 21,700 | 4.5% | 
| 26 | Mountain Brook | 21,094 | 0.2% | 
| 27 | Oxford | 20,800 | 1.0% | 
| 28 | Albertville | 20,115 | 1.6% | 
| 29 | Daphne | 19,542 | 1.7% | 
| 30 | Trussville | 19,208 | 3.3% | 
| 31 | Selma | 18,278 | -1.2% | 
| 32 | Fairhope | 17,550 | 3.6% | 
| 33 | Millbrook | 16,893 | 4.6% | 
| 34 | Talladega | 16,735 | -0.4% | 
| 35 | Hueytown | 15,846 | -0.3% | 
| 36 | Center Point | 15,519 | -0.3% | 
| 37 | Cullman | 15,302 | 0.7% | 
| 38 | Helena | 15,182 | 3.8% | 
| 39 | Troy | 15,142 | 0.8% | 
| 40 | Alexander City | 15,114 | -0.1% | 
| 41 | Scottsboro | 14,879 | 0.1% | 
| 42 | Ozark | 14,588 | -0.4% | 
| 43 | Eufaula | 14,521 | 0.4% | 
| 44 | Foley | 14,197 | 5.6% | 
| 45 | Fort Payne | 14,165 | 1.0% | 
| 46 | Jasper | 14,029 | -0.1% | 
| 47 | Hartselle | 13,955 | 1.4% | 
| 48 | Moody | 13,845 | 6.0% | 
| 49 | Gardendale | 13,720 | 1.7% | 
| 50 | Pell City | 13,112 | 3.4% | 
| 51 | Muscle Shoals | 13,073 | 0.7% | 
| 52 | Saraland | 13,043 | 0.7% | 
| 53 | Sylacauga | 13,040 | 0.0% | 
| 54 | Leeds | 11,473 | 1.0% | 
| 55 | Fairfield | 11,336 | -0.9% | 
| 56 | Tuskegee | 11,063 | -1.0% | 
| 57 | Calera | 10,984 | 13.3% | 
| 58 | Pleasant Grove | 10,287 | 0.3% | 
| 59 | Gulf Shores | 10,268 | 6.7% | 
| 60 | Jacksonville | 10,183 | 2.1% | 
| 61 | Atmore | 10,109 | -0.2% | 
| 62 | Valley | 9,841 | 0.4% | 
| 63 | Irondale | 9,778 | -0.0% | 
| 64 | Rainbow City | 9,387 | 1.2% | 
| 65 | Sheffield | 9,031 | -0.7% | 
| 66 | Russellville | 8,961 | -0.0% | 
| 67 | Clanton | 8,929 | 1.2% | 
| 68 | Andalusia | 8,863 | 0.0% | 
| 69 | Guntersville | 8,601 | 1.2% | 
| 70 | Boaz | 8,501 | 1.0% | 
| 71 | Southside | 8,495 | 2.1% | 
| 72 | Clay | 8,409 | -0.4% | 
| 73 | Tuscumbia | 8,374 | 0.5% | 
| 74 | Bay Minette | 8,342 | 0.7% | 
| 75 | Arab | 8,272 | 1.2% | 
| 76 | Wetumpka | 7,798 | 3.3% | 
| 77 | Spanish Fort | 7,539 | 3.6% | 
| 78 | Demopolis | 7,300 | -0.8% | 
| 79 | Oneonta | 7,085 | 2.6% | 
| 80 | Lanett | 7,045 | -1.3% | 
| 81 | Fultondale | 6,993 | 0.6% | 
| 82 | Greenville | 6,929 | -0.5% | 
| 83 | Pinson | 6,899 | -0.4% | 
| 84 | Roanoke | 6,649 | 0.1% | 
| 85 | Attalla | 6,587 | -0.4% | 
| 86 | Opp | 6,524 | -0.5% | 
| 87 | Tarrant | 6,518 | -0.8% | 
| 88 | Montevallo | 6,447 | 2.6% | 
| 89 | Hamilton | 6,314 | -0.8% | 
| 90 | Monroeville | 6,285 | -1.0% | 
| 91 | Orange Beach | 6,221 | 5.2% | 
| 92 | Lincoln | 6,088 | 2.5% | 
| 93 | Satsuma | 6,032 | 0.6% | 
| 94 | Chickasaw | 5,934 | -0.7% | 
| 95 | Glencoe | 5,321 | 0.5% | 
| 96 | Brewton | 5,240 | -0.5% | 
| 97 | Tallassee | 5,237 | 0.6% | 
| 98 | Midfield | 5,208 | -0.8% | 
| 99 | Robertsdale | 5,120 | 3.3% | 
| 100 | Jackson | 5,096 | -0.6% | 
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