| Rank | City | 1990 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Baltimore | 736,014 | -0.7% |
| 2 | Rockville | 45,181 | – |
| 3 | Frederick | 40,504 | – |
| 4 | Gaithersburg | 39,567 | – |
| 5 | Bowie | 37,830 | – |
| 6 | Hagerstown | 35,939 | – |
| 7 | Annapolis | 32,527 | – |
| 8 | Cumberland | 24,858 | – |
| 9 | College Park | 23,705 | – |
| 10 | Salisbury | 20,659 | – |
| 11 | Greenbelt | 20,558 | – |
| 12 | Laurel | 19,309 | – |
| 13 | Takoma Park | 17,277 | – |
| 14 | Hyattsville | 13,934 | – |
| 15 | Aberdeen | 13,504 | – |
| 16 | Westminster | 12,878 | – |
| 17 | New Carrollton | 12,021 | – |
| 18 | Cambridge | 11,572 | – |
| 19 | Easton | 9,601 | – |
| 20 | Frostburg | 9,099 | – |
| 21 | Bel Air | 9,008 | – |
| 22 | Havre de Grace | 8,996 | – |
| 23 | Elkton | 8,548 | – |
| 24 | Mount Rainier | 8,048 | – |
| 25 | Bladensburg | 8,032 | – |
| 26 | District Heights | 6,337 | – |
| 27 | Cheverly | 6,105 | – |
| 28 | La Plata | 5,993 | – |
| 29 | Seat Pleasant | 5,354 | – |
| 30 | Ocean City | 5,148 | – |
| 31 | Brunswick | 5,091 | – |
| 32 | Riverdale | 5,087 | – |
| 33 | Glenarden | 5,068 | – |
| 34 | Walkersville | 4,118 | – |
| 35 | Chestertown | 3,996 | – |
| 36 | Pocomoke City | 3,945 | – |
| 37 | Mount Airy | 3,897 | – |
| 38 | Poolesville | 3,802 | – |
| 39 | Indian Head | 3,742 | – |
| 40 | Taneytown | 3,705 | – |
| 41 | Capitol Heights | 3,630 | – |
| 42 | Denton | 3,541 | – |
| 43 | Fruitland | 3,511 | – |
| 44 | Thurmont | 3,442 | – |
| 45 | Crisfield | 3,312 | – |
| 46 | Berwyn Heights | 2,952 | – |
| 47 | Manchester | 2,946 | – |
| 48 | Chevy Chase | 2,927 | – |
| 49 | Brentwood | 2,875 | – |
| 50 | Forest Heights | 2,855 | – |
| 51 | Berlin | 2,710 | – |
| 52 | Hampstead | 2,658 | – |
| 53 | Westernport | 2,645 | – |
| 54 | Williamsport | 2,577 | – |
| 55 | Boonsboro | 2,540 | – |
| 56 | Perryville | 2,444 | – |
| 57 | Chesapeake Beach | 2,369 | – |
| 58 | Federalsburg | 2,348 | – |
| 59 | Sykesville | 2,328 | – |
| 60 | University Park | 2,236 | – |
| 61 | Centreville | 2,148 | – |
| 62 | Snow Hill | 2,128 | – |
| 63 | Chevy Chase Village | 2,058 | – |
| 64 | Mountain Lake Park | 2,040 | – |
| 65 | Hancock | 1,956 | – |
| 66 | Oakland | 1,927 | – |
| 67 | Emmitsburg | 1,902 | – |
| 68 | Princess Anne | 1,874 | – |
| 69 | North East | 1,842 | – |
| 70 | Middletown | 1,830 | – |
| 71 | Kensington | 1,742 | – |
| 72 | Hurlock | 1,697 | – |
| 73 | Leonardtown | 1,690 | – |
| 74 | Rock Hall | 1,584 | – |
| 75 | Delmar | 1,583 | – |
| 76 | Greensboro | 1,465 | – |
| 77 | Landover Hills | 1,460 | – |
| 78 | Morningside | 1,392 | – |
| 79 | Lonaconing | 1,377 | – |
| 80 | St. Michaels | 1,319 | – |
| 81 | Rising Sun | 1,313 | – |
| 82 | Smithsburg | 1,308 | – |
| 83 | Cottage City | 1,273 | – |
| 84 | Fairmount Heights | 1,253 | – |
| 85 | Colmar Manor | 1,238 | – |
| 86 | North Beach | 1,208 | – |
| 87 | Funkstown | 1,116 | – |
| 88 | Somerset | 1,062 | – |
| 89 | Ridgely | 1,011 | – |
| 90 | Trappe | 988 | – |
| 91 | Union Bridge | 952 | – |
| 92 | Edmonston | 933 | – |
| 93 | Clear Spring | 898 | – |
| 94 | Garrett Park | 882 | – |
| 95 | Martin's Additions | 843 | – |
| 96 | Chevy Chase View | 842 | – |
| 97 | Port Deposit | 820 | – |
| 98 | Chesapeake City | 789 | – |
| 99 | New Windsor | 769 | – |
| 100 | Upper Marlboro | 750 | – |
In 1990, the largest city in Maryland was Baltimore, with a population of 736,014. The second largest city is Rockville, with a population of 45,181.
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