| Rank | City | 2000 Population | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Omaha | 409,868 | 1.4% |
| 2 | Lincoln | 227,701 | 1.7% |
| 3 | Bellevue | 44,515 | 1.3% |
| 4 | Grand Island | 43,548 | 1.0% |
| 5 | Kearney | 27,508 | 1.0% |
| 6 | Fremont | 25,262 | 0.6% |
| 7 | Hastings | 24,882 | 0.8% |
| 8 | North Platte | 23,930 | 0.5% |
| 9 | Norfolk | 23,387 | 0.9% |
| 10 | Columbus | 21,094 | 0.7% |
| 11 | Papillion | 18,630 | 3.3% |
| 12 | Scottsbluff | 14,892 | 0.6% |
| 13 | Beatrice | 12,554 | 0.1% |
| 14 | South Sioux City | 11,978 | 2.0% |
| 15 | La Vista | 11,812 | 1.7% |
| 16 | Lexington | 10,033 | 4.0% |
| 17 | Alliance | 8,921 | -1.0% |
| 18 | York | 8,079 | 0.1% |
| 19 | McCook | 7,987 | -0.2% |
| 20 | Gering | 7,741 | -0.4% |
| 21 | Blair | 7,518 | 0.7% |
| 22 | Nebraska City | 7,224 | 0.7% |
| 23 | Plattsmouth | 6,900 | 0.3% |
| 24 | Seward | 6,508 | 1.1% |
| 25 | Sidney | 6,365 | 0.5% |
| 26 | Ralston | 6,264 | -0.2% |
| 27 | Crete | 6,103 | 1.9% |
| 28 | Holdrege | 5,649 | 0.0% |
| 29 | Chadron | 5,625 | -0.0% |
| 30 | Wayne | 5,558 | 0.6% |
| 31 | Schuyler | 5,420 | 2.6% |
| 32 | Ogallala | 5,112 | -0.0% |
| 33 | Falls City | 4,656 | -0.4% |
| 34 | Fairbury | 4,312 | -0.2% |
| 35 | Aurora | 4,224 | 0.9% |
| 36 | Cozad | 4,186 | 0.7% |
| 37 | Wahoo | 4,186 | 1.3% |
| 38 | O'Neill | 3,716 | -0.8% |
| 39 | West Point | 3,658 | 1.1% |
| 40 | Gothenburg | 3,627 | 1.0% |
| 41 | Auburn | 3,536 | 0.3% |
| 42 | Broken Bow | 3,502 | -0.8% |
| 43 | Central City | 2,995 | 0.3% |
| 44 | Minden | 2,988 | 0.8% |
| 45 | Gretna | 2,976 | 2.7% |
| 46 | Valentine | 2,884 | 0.0% |
| 47 | David City | 2,611 | -0.0% |
| 48 | Kimball | 2,545 | -0.4% |
| 49 | Waverly | 2,479 | 2.9% |
| 50 | Madison | 2,362 | 0.9% |
| 51 | Ashland | 2,275 | 0.7% |
| 52 | Ord | 2,267 | -1.2% |
| 53 | Geneva | 2,224 | -0.4% |
| 54 | St. Paul | 2,221 | 0.7% |
| 55 | Milford | 2,114 | 0.9% |
| 56 | Superior | 2,041 | -1.8% |
| 57 | Imperial | 1,972 | -0.2% |
| 58 | Tekamah | 1,892 | 0.2% |
| 59 | Ainsworth | 1,858 | -0.4% |
| 60 | Mitchell | 1,831 | 0.5% |
| 61 | Dakota City | 1,822 | 2.1% |
| 62 | Valley | 1,795 | 0.1% |
| 63 | Albion | 1,783 | -0.9% |
| 64 | Pierce | 1,778 | 0.9% |
| 65 | Syracuse | 1,775 | 0.4% |
| 66 | Wilber | 1,766 | 1.4% |
| 67 | Gibbon | 1,760 | 1.4% |
| 68 | Gordon | 1,748 | -0.5% |
| 69 | Tecumseh | 1,711 | -0.0% |
| 70 | Neligh | 1,649 | -0.6% |
| 71 | Wymore | 1,649 | 0.0% |
| 72 | Hartington | 1,639 | -0.2% |
| 73 | Stanton | 1,619 | 0.0% |
| 74 | Bridgeport | 1,592 | 0.0% |
| 75 | Hebron | 1,561 | -1.3% |
| 76 | Springfield | 1,455 | -0.1% |
| 77 | Sutton | 1,447 | 0.7% |
| 78 | Wakefield | 1,403 | 1.9% |
| 79 | Fullerton | 1,383 | -0.8% |
| 80 | Ravenna | 1,370 | -0.0% |
| 81 | Oakland | 1,365 | 0.8% |
| 82 | Plainview | 1,347 | 0.0% |
| 83 | Wisner | 1,266 | 0.1% |
| 84 | Creighton | 1,263 | 0.3% |
| 85 | Atkinson | 1,245 | -1.1% |
| 86 | Bayard | 1,244 | – |
| 87 | Terrytown | 1,225 | 8.6% |
| 88 | Stromsburg | 1,225 | -0.3% |
| 89 | Yutan | 1,215 | 0.2% |
| 90 | North Bend | 1,215 | -0.3% |
| 91 | Grant | 1,215 | -0.2% |
| 92 | Wood River | 1,207 | 0.4% |
| 93 | Alma | 1,207 | -0.1% |
| 94 | Arlington | 1,202 | 0.1% |
| 95 | Friend | 1,173 | 0.2% |
| 96 | Sutherland | 1,166 | 1.0% |
| 97 | Battle Creek | 1,156 | 1.4% |
| 98 | Pender | 1,146 | -0.5% |
| 99 | Shelton | 1,142 | 1.8% |
| 100 | Red Cloud | 1,127 | -1.0% |
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