| Rank | City | 1840 Population | 
|---|---|---|
| 1 | Washington | 23,364 | 
| 2 | Alexandria | 8,459 | 
| 3 | Georgetown | 7,312 | 
In 1840, the largest city in District of Columbia was Washington, with a population of 23,364. The second largest city is Alexandria, with a population of 8,459.
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