| Rank | City | 2023 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Summerset | 3,052 | 0.9% |
| 2 | Harrisburg | 8,447 | 11.1% |
| 3 | Tea | 6,916 | 10.4% |
| 4 | Piedmont | 1,032 | 3.0% |
| 5 | Box Elder | 12,558 | 2.6% |
| 6 | Akaska | 79 | 4.0% |
| 7 | Aurora | 1,133 | 3.9% |
| 8 | Brandon | 11,104 | 0.5% |
| 9 | Cottonwood | 11 | – |
| 10 | Hartford | 3,428 | 1.2% |
| 11 | Naples | 41 | 5.3% |
| 12 | Sioux Falls | 202,016 | 2.2% |
| 13 | Baltic | 1,317 | 2.8% |
| 14 | Crooks | 1,382 | 1.1% |
| 15 | Worthing | 940 | 0.5% |
| 16 | Volga | 2,272 | 4.0% |
| 17 | Spearfish | 12,924 | 2.9% |
| 18 | Quinn | 64 | 0.8% |
| 19 | Lake Norden | 595 | -0.1% |
| 20 | Eagle Butte | 1,241 | -0.8% |
| 21 | Brentford | 86 | -1.1% |
| 22 | North Sioux City | 3,026 | -0.3% |
| 23 | Vilas | 25 | -1.9% |
| 24 | Pickstown | 222 | -2.2% |
| 25 | Dell Rapids | 3,927 | -0.7% |
| 26 | Rapid City | 78,716 | 2.9% |
| 27 | Plankinton | 774 | – |
| 28 | Brookings | 24,003 | 1.2% |
| 29 | Mission | 1,145 | – |
| 30 | Fruitdale | 77 | 2.7% |
| 31 | Huron | 14,539 | 1.0% |
| 32 | Springfield | 1,915 | -3.4% |
| 33 | Elk Point | 2,101 | -0.8% |
| 34 | Hill City | 955 | 3.7% |
| 35 | Belle Fourche | 5,856 | 2.3% |
| 36 | Hermosa | 379 | 0.5% |
| 37 | Lennox | 2,466 | 0.8% |
| 38 | Bryant | 475 | 1.5% |
| 39 | Parker | 1,225 | 0.7% |
| 40 | Verdon | 7 | 8.0% |
| 41 | Wetonka | 14 | 3.8% |
| 42 | Vermillion | 11,931 | 1.1% |
| 43 | Alexandria | 656 | 4.8% |
| 44 | Dupree | 491 | -0.7% |
| 45 | Yankton | 15,557 | 0.5% |
| 46 | Camp Crook | 63 | -13.9% |
| 47 | Timber Lake | 503 | -0.8% |
| 48 | Watertown | 23,031 | 0.9% |
| 49 | Elkton | 768 | 1.1% |
| 50 | Warner | 474 | -0.7% |
| 51 | Colman | 643 | 0.8% |
| 52 | Humboldt | 594 | 1.5% |
| 53 | Veblen | 316 | -1.1% |
| 54 | Columbia | 157 | 0.3% |
| 55 | Aberdeen | 28,157 | -0.5% |
| 56 | Northville | 137 | -1.1% |
| 57 | Wolsey | 464 | 0.7% |
| 58 | Florence | 332 | 0.5% |
| 59 | Estelline | 752 | -2.2% |
| 60 | Sturgis | 7,167 | 0.5% |
| 61 | Valley Springs | 877 | 0.1% |
| 62 | Whitewood | 930 | 2.3% |
| 63 | Emery | 483 | 4.5% |
| 64 | Ethan | 360 | 0.6% |
| 65 | Wallace | 94 | – |
| 66 | Hayti | 400 | 1.4% |
| 67 | Colton | 720 | -1.0% |
| 68 | Iroquois | 297 | -0.5% |
| 69 | Mitchell | 15,611 | -0.2% |
| 70 | Castlewood | 712 | 2.0% |
| 71 | Chamberlain | 2,492 | 0.1% |
| 72 | Avon | 596 | 2.7% |
| 73 | Beresford | 2,129 | -1.1% |
| 74 | Fort Pierre | 2,155 | 0.8% |
| 75 | Faulkton | 822 | -0.4% |
| 76 | Brandt | 118 | 0.9% |
| 77 | Centerville | 947 | 1.7% |
| 78 | Pierpont | 126 | -0.4% |
| 79 | Virgil | 26 | – |
| 80 | New Witten | 53 | -1.8% |
| 81 | Mission Hill | 190 | 0.5% |
| 82 | Toronto | 199 | 0.5% |
| 83 | Gary | 236 | -1.2% |
| 84 | Garretson | 1,191 | -1.3% |
| 85 | Groton | 1,377 | -0.3% |
| 86 | Fulton | 87 | 5.6% |
| 87 | White | 538 | 0.8% |
| 88 | Hitchcock | 109 | 0.9% |
| 89 | Ipswich | 948 | 1.6% |
| 90 | Pierre | 13,962 | -0.2% |
| 91 | Irene | 433 | 0.7% |
| 92 | Nisland | 211 | 1.0% |
| 93 | Montrose | 465 | 1.1% |
| 94 | Nunda | 47 | 1.1% |
| 95 | Stockholm | 105 | -0.9% |
| 96 | Lead | 3,017 | 0.7% |
| 97 | White Lake | 405 | 1.5% |
| 98 | Freeman | 1,308 | -0.7% |
| 99 | Flandreau | 2,358 | -0.3% |
| 100 | Dimock | 150 | 0.7% |
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