| Rank | City | 2022 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Summerset | 3,027 | 1.1% |
| 2 | Harrisburg | 7,638 | 11.6% |
| 3 | Box Elder | 12,310 | 3.2% |
| 4 | Tea | 6,256 | 10.2% |
| 5 | Piedmont | 965 | -0.7% |
| 6 | Akaska | 74 | 1.4% |
| 7 | Aurora | 1,071 | 2.1% |
| 8 | Brandon | 10,950 | -0.3% |
| 9 | Cottonwood | 11 | – |
| 10 | Hartford | 3,376 | 0.9% |
| 11 | Sioux Falls | 196,528 | 1.7% |
| 12 | Crooks | 1,356 | 0.3% |
| 13 | Worthing | 928 | -0.2% |
| 14 | Camp Crook | 85 | – |
| 15 | Baltic | 1,265 | 1.5% |
| 16 | Naples | 37 | – |
| 17 | Volga | 2,151 | 2.5% |
| 18 | Quinn | 63 | – |
| 19 | Spearfish | 12,358 | 1.3% |
| 20 | Lake Norden | 607 | 1.8% |
| 21 | Eagle Butte | 1,260 | – |
| 22 | Vilas | 26 | – |
| 23 | Pickstown | 225 | -3.0% |
| 24 | Brentford | 86 | -2.3% |
| 25 | Dell Rapids | 3,953 | -0.7% |
| 26 | North Sioux City | 3,015 | -0.9% |
| 27 | Springfield | 2,053 | – |
| 28 | Plankinton | 778 | 0.5% |
| 29 | Rapid City | 76,184 | 2.5% |
| 30 | Mission | 1,146 | 0.1% |
| 31 | Brookings | 23,577 | 0.6% |
| 32 | Elk Point | 2,124 | -0.5% |
| 33 | Huron | 14,231 | -0.1% |
| 34 | Fruitdale | 74 | 1.4% |
| 35 | Hermosa | 373 | -0.5% |
| 36 | Belle Fourche | 5,699 | 1.7% |
| 37 | Hill City | 922 | 3.8% |
| 38 | Lennox | 2,405 | -0.8% |
| 39 | Dupree | 497 | -0.2% |
| 40 | Bryant | 463 | 0.4% |
| 41 | Timber Lake | 513 | 0.4% |
| 42 | Estelline | 786 | -0.1% |
| 43 | Parker | 1,208 | -0.1% |
| 44 | Vermillion | 11,802 | 1.1% |
| 45 | Yankton | 15,453 | 0.4% |
| 46 | Veblen | 321 | -0.6% |
| 47 | Warner | 478 | -0.6% |
| 48 | Aberdeen | 28,324 | -0.4% |
| 49 | Watertown | 22,722 | 0.5% |
| 50 | Northville | 138 | -1.4% |
| 51 | Columbia | 157 | 0.6% |
| 52 | Elkton | 753 | 0.3% |
| 53 | Colman | 629 | -0.6% |
| 54 | Florence | 329 | – |
| 55 | Sturgis | 7,107 | 0.2% |
| 56 | Wolsey | 457 | -0.2% |
| 57 | Valley Springs | 871 | -0.5% |
| 58 | Wallace | 94 | – |
| 59 | Colton | 725 | -1.2% |
| 60 | Wetonka | 13 | – |
| 61 | Ethan | 356 | – |
| 62 | New Witten | 55 | – |
| 63 | Humboldt | 568 | -1.6% |
| 64 | Iroquois | 298 | -0.7% |
| 65 | Beresford | 2,145 | -1.5% |
| 66 | Mitchell | 15,631 | -0.3% |
| 67 | Alexandria | 605 | 1.3% |
| 68 | Whitewood | 898 | 1.1% |
| 69 | Faulkton | 829 | 0.1% |
| 70 | Hayti | 388 | -0.3% |
| 71 | Fort Pierre | 2,162 | 2.0% |
| 72 | Chamberlain | 2,468 | -0.7% |
| 73 | Virgil | 26 | – |
| 74 | Gary | 239 | -1.2% |
| 75 | Castlewood | 690 | 0.9% |
| 76 | Kennebec | 297 | 4.9% |
| 77 | Pierpont | 125 | -1.6% |
| 78 | Woonsocket | 741 | 2.1% |
| 79 | Garretson | 1,202 | -1.6% |
| 80 | Stockholm | 108 | 0.9% |
| 81 | Groton | 1,382 | -0.2% |
| 82 | Mission Hill | 187 | -0.5% |
| 83 | Emery | 449 | 1.6% |
| 84 | Brandt | 115 | -0.9% |
| 85 | Toronto | 195 | -1.0% |
| 86 | Centerville | 922 | 0.8% |
| 87 | Avon | 569 | 0.7% |
| 88 | Pierre | 14,000 | -0.2% |
| 89 | Freeman | 1,322 | -0.4% |
| 90 | Nunda | 47 | 2.2% |
| 91 | Verdon | 6 | – |
| 92 | Langford | 290 | – |
| 93 | Nisland | 209 | 1.0% |
| 94 | White | 529 | – |
| 95 | Flandreau | 2,354 | -0.7% |
| 96 | Hitchcock | 107 | – |
| 97 | Irene | 427 | – |
| 98 | Summit | 282 | -1.4% |
| 99 | Custer | 1,949 | -0.9% |
| 100 | New Effington | 230 | -1.7% |
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