| Rank | City | 2007 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Sioux Falls | 148,244 | 2.8% |
| 2 | Rapid City | 63,278 | 0.9% |
| 3 | Aberdeen | 24,788 | -0.3% |
| 4 | Watertown | 20,370 | 0.1% |
| 5 | Brookings | 19,435 | 0.5% |
| 6 | Mitchell | 14,863 | 0.3% |
| 7 | Pierre | 13,967 | 0.1% |
| 8 | Yankton | 13,687 | 0.2% |
| 9 | Huron | 10,806 | -1.5% |
| 10 | Vermillion | 10,200 | -0.1% |
| 11 | Spearfish | 9,755 | 2.0% |
| 12 | Brandon | 7,110 | 3.4% |
| 13 | Madison | 6,244 | -0.7% |
| 14 | Sturgis | 6,151 | -0.8% |
| 15 | Belle Fourche | 4,816 | 0.0% |
| 16 | Canton | 4,155 | 4.8% |
| 17 | Hot Springs | 4,112 | -0.1% |
| 18 | Box Elder | 3,644 | 1.0% |
| 19 | Tea | 3,548 | 12.1% |
| 20 | Milbank | 3,266 | -1.8% |
| 21 | Mobridge | 3,226 | -1.6% |
| 22 | Harrisburg | 3,205 | 21.7% |
| 23 | Dell Rapids | 3,021 | 0.1% |
| 24 | Lead | 2,889 | -0.7% |
| 25 | Winner | 2,848 | -1.5% |
| 26 | Lennox | 2,765 | 5.1% |
| 27 | North Sioux City | 2,502 | 1.5% |
| 28 | Sisseton | 2,473 | -0.5% |
| 29 | Redfield | 2,323 | -1.4% |
| 30 | Flandreau | 2,261 | -0.8% |
| 31 | Chamberlain | 2,230 | -0.8% |
| 32 | Beresford | 2,162 | 1.3% |
| 33 | Fort Pierre | 2,076 | 0.3% |
| 34 | Custer | 2,043 | 0.6% |
| 35 | Hartford | 1,976 | 0.8% |
| 36 | Elk Point | 1,849 | 1.2% |
| 37 | Webster | 1,735 | -1.9% |
| 38 | Volga | 1,523 | 0.7% |
| 39 | Wagner | 1,518 | -1.0% |
| 40 | Parkston | 1,506 | -1.7% |
| 41 | Springfield | 1,498 | -0.7% |
| 42 | Groton | 1,377 | 0.3% |
| 43 | Miller | 1,347 | -2.1% |
| 44 | Salem | 1,343 | -0.6% |
| 45 | Platte | 1,297 | -0.8% |
| 46 | Deadwood | 1,296 | -1.0% |
| 47 | Britton | 1,256 | -0.9% |
| 48 | Clear Lake | 1,228 | -1.3% |
| 49 | Gregory | 1,202 | -2.4% |
| 50 | Lemmon | 1,198 | -2.4% |
| 51 | Freeman | 1,180 | -1.8% |
| 52 | Gettysburg | 1,124 | -2.8% |
| 53 | Tyndall | 1,122 | -1.6% |
| 54 | Clark | 1,099 | -2.5% |
| 55 | De Smet | 1,091 | -1.6% |
| 56 | Garretson | 1,090 | -1.1% |
| 57 | Worthing | 1,061 | 10.0% |
| 58 | Crooks | 1,048 | 3.2% |
| 59 | Martin | 1,001 | -1.2% |
| 60 | Parker | 975 | -1.1% |
| 61 | Eureka | 967 | -2.1% |
| 62 | Mission | 956 | 0.9% |
| 63 | Eagle Butte | 952 | 0.7% |
| 64 | Arlington | 925 | -1.2% |
| 65 | Howard | 916 | -2.6% |
| 66 | Alcester | 883 | 0.1% |
| 67 | Baltic | 881 | 1.1% |
| 68 | Hill City | 878 | 1.9% |
| 69 | Wessington Springs | 868 | -2.4% |
| 70 | Ipswich | 857 | -1.5% |
| 71 | Centerville | 842 | -1.2% |
| 72 | Whitewood | 831 | -0.3% |
| 73 | Marion | 817 | -1.4% |
| 74 | Scotland | 806 | -1.6% |
| 75 | Wall | 806 | -0.2% |
| 76 | Edgemont | 795 | -1.3% |
| 77 | Viborg | 780 | -1.1% |
| 78 | Lake Andes | 769 | -1.0% |
| 79 | St. Francis | 765 | 1.4% |
| 80 | Valley Springs | 764 | -0.7% |
| 81 | Highmore | 753 | -1.9% |
| 82 | Philip | 741 | -2.8% |
| 83 | McLaughlin | 722 | -1.0% |
| 84 | Armour | 688 | -2.0% |
| 85 | Faulkton | 676 | -2.4% |
| 86 | Kimball | 675 | -1.6% |
| 87 | New Underwood | 674 | 1.3% |
| 88 | Menno | 669 | -1.3% |
| 89 | Selby | 669 | -1.5% |
| 90 | Canistota | 666 | -0.9% |
| 91 | Estelline | 657 | -0.5% |
| 92 | Castlewood | 656 | -0.3% |
| 93 | Lake Preston | 655 | -1.9% |
| 94 | Woonsocket | 651 | -1.6% |
| 95 | Onida | 647 | -2.1% |
| 96 | Kadoka | 644 | -1.5% |
| 97 | Tripp | 644 | -1.7% |
| 98 | Alexandria | 642 | 2.1% |
| 99 | Elkton | 636 | -1.0% |
| 100 | Presho | 623 | 0.9% |
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