| Rank | City | 2000 Population |
|---|---|---|
| 1 | Franklin | 29,633 |
| 2 | Northampton | 28,974 |
| 3 | Agawam | 28,184 |
| 4 | West Springfield | 27,936 |
| 5 | Gardner | 20,839 |
| 6 | Easthampton | 16,003 |
| 7 | Brockton | 94,455 |
| 8 | New Bedford | 93,896 |
| 9 | Fall River | 92,116 |
| 10 | Somerville | 77,510 |
| 11 | Waltham | 59,214 |
| 12 | Taunton | 56,119 |
| 13 | Medford | 55,662 |
| 14 | Barnstable Town | 48,054 |
| 15 | Pittsfield | 45,704 |
| 16 | Methuen | 43,952 |
| 17 | Attleboro | 41,954 |
| 18 | Holyoke | 39,883 |
| 19 | Beverly | 39,842 |
| 20 | Fitchburg | 39,010 |
| 21 | Woburn | 37,420 |
| 22 | Marlborough | 36,612 |
| 23 | Watertown | 33,003 |
| 24 | North Adams | 14,651 |
| 25 | Gloucester | 30,357 |
| 26 | Melrose | 27,183 |
| 27 | Newburyport | 17,183 |
| 28 | Chelsea | 35,105 |
| 29 | Boston | 590,433 |
| 30 | Worcester | 172,500 |
| 31 | Springfield | 151,895 |
| 32 | Lowell | 105,181 |
| 33 | Cambridge | 101,683 |
| 34 | Lynn | 89,210 |
| 35 | Quincy | 88,351 |
| 36 | Newton | 83,788 |
| 37 | Lawrence | 72,176 |
| 38 | Haverhill | 59,113 |
| 39 | Malden | 56,296 |
| 40 | Chicopee | 54,735 |
| 41 | Peabody | 48,376 |
| 42 | Revere | 47,383 |
| 43 | Leominster | 41,469 |
| 44 | Salem | 40,450 |
| 45 | Westfield | 40,084 |
| 46 | Everett | 38,291 |
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