| Rank | City | 2003 Population (Est.) | Growth Rate (Annual) |
|---|---|---|---|
| 1 | Tea | 2,109 | 8.5% |
| 2 | Worthing | 685 | 6.8% |
| 3 | Harrisburg | 1,077 | 4.5% |
| 4 | Brandon | 6,183 | 3.2% |
| 5 | Sioux Falls | 133,281 | 3.0% |
| 6 | Baltic | 871 | 2.9% |
| 7 | Fulton | 91 | 2.9% |
| 8 | Alexandria | 598 | 2.8% |
| 9 | Farmer | 19 | 2.7% |
| 10 | Emery | 465 | 2.7% |
| 11 | Crooks | 907 | 2.3% |
| 12 | Quinn | 46 | 2.2% |
| 13 | Batesland | 93 | 2.2% |
| 14 | Eagle Butte | 950 | 2.1% |
| 15 | Hill City | 815 | 2.0% |
| 16 | Canton | 3,256 | 1.8% |
| 17 | Humboldt | 547 | 1.8% |
| 18 | Elk Point | 1,778 | 1.8% |
| 19 | Pickstown | 175 | 1.8% |
| 20 | Fairview | 98 | 1.6% |
| 21 | Lennox | 2,117 | 1.5% |
| 22 | St. Francis | 726 | 1.5% |
| 23 | Volga | 1,498 | 1.4% |
| 24 | Wasta | 77 | 1.3% |
| 25 | Summerset | 344 | 1.3% |
| 26 | Hartford | 1,933 | 1.2% |
| 27 | Keystone | 318 | 1.1% |
| 28 | Mission | 924 | 1.0% |
| 29 | Presho | 603 | 0.9% |
| 30 | Custer | 2,006 | 0.9% |
| 31 | Reliance | 210 | 0.7% |
| 32 | Warner | 425 | 0.7% |
| 33 | North Sioux City | 2,318 | 0.7% |
| 34 | Morristown | 83 | 0.6% |
| 35 | Hudson | 409 | 0.6% |
| 36 | Brookings | 19,064 | 0.6% |
| 37 | Oacoma | 437 | 0.6% |
| 38 | Dell Rapids | 3,030 | 0.6% |
| 39 | Rapid City | 60,683 | 0.5% |
| 40 | Box Elder | 3,464 | 0.5% |
| 41 | Dupree | 429 | 0.5% |
| 42 | Wall | 825 | 0.4% |
| 43 | Bruce | 275 | 0.4% |
| 44 | New Underwood | 628 | 0.3% |
| 45 | Buffalo Gap | 165 | 0.3% |
| 46 | Montrose | 467 | 0.3% |
| 47 | Valley Springs | 799 | 0.3% |
| 48 | Piedmont | 279 | 0.2% |
| 49 | Florence | 300 | 0.2% |
| 50 | Estelline | 680 | 0.1% |
| 51 | Alcester | 881 | 0.1% |
| 52 | Mitchell | 14,642 | 0.1% |
| 53 | Canistota | 704 | 0.1% |
| 54 | Jefferson | 586 | 0.1% |
| 55 | Spearfish | 8,693 | 0.1% |
| 56 | Wetonka | 12 | – |
| 57 | Fairburn | 80 | – |
| 58 | White Rock | 18 | – |
| 59 | South Shore | 269 | – |
| 60 | Wallace | 86 | – |
| 61 | Kranzburg | 185 | – |
| 62 | Hillsview | 3 | – |
| 63 | Cottonwood | 6 | – |
| 64 | Verdon | 6 | – |
| 65 | Albee | 10 | – |
| 66 | Yankton | 13,479 | -0.1% |
| 67 | Pierre | 13,869 | -0.1% |
| 68 | Beresford | 1,999 | -0.1% |
| 69 | Castlewood | 665 | -0.2% |
| 70 | Vermillion | 10,222 | -0.1% |
| 71 | Watertown | 20,199 | -0.2% |
| 72 | Hermosa | 313 | -0.2% |
| 73 | Whitewood | 841 | -0.2% |
| 74 | Salem | 1,386 | -0.2% |
| 75 | Henry | 267 | -0.2% |
| 76 | Nisland | 209 | -0.2% |
| 77 | McLaughlin | 763 | -0.3% |
| 78 | Hayti | 364 | -0.3% |
| 79 | Sinai | 127 | -0.4% |
| 80 | Rosholt | 417 | -0.4% |
| 81 | Trent | 251 | -0.4% |
| 82 | Sturgis | 6,428 | -0.4% |
| 83 | Isabel | 237 | -0.4% |
| 84 | Westport | 124 | -0.4% |
| 85 | Pringle | 124 | -0.4% |
| 86 | Groton | 1,342 | -0.4% |
| 87 | Stratford | 95 | -0.5% |
| 88 | Utica | 85 | -0.6% |
| 89 | Claire City | 84 | -0.6% |
| 90 | Sherman | 86 | -0.6% |
| 91 | Timber Lake | 437 | -0.6% |
| 92 | Lead | 2,987 | -0.6% |
| 93 | Lesterville | 158 | -0.6% |
| 94 | Ravinia | 78 | -0.6% |
| 95 | Goodwin | 158 | -0.6% |
| 96 | McIntosh | 215 | -0.7% |
| 97 | Oelrichs | 142 | -0.7% |
| 98 | Kennebec | 283 | -0.7% |
| 99 | Bushnell | 74 | -0.7% |
| 100 | Colton | 654 | -0.7% |
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